Objetive. Human teeth have been commonly used for in vitro and in situ studies. Cu­rrently, other animals’ teeth have been purposed for dental research to overcome human teeth’ problematic availability. This study aimed to investigate the enamel and dentin from human, bovine, and ovine teeth concerning the microhardness, organic, and inorganic contents via micro-Raman spectroscopy. Methods. Human, bovine, and ovine teeth were divided according to their type and age into seven groups: Ovine; Bovine-12 months; Bovine-24 months; Bovine-36 months; Bovine-48 months; Bovine-+60 months; Human (control). The enamel’s microhardness (superficial and deep) and dentin (superficial, middle, and deep) were analyzed. The calcium/phosphate ratio and amide contents were determined by micro-Raman spectroscopy. Results. Overall, the microhardness of human enamel was superior to the other species. De...

Objetives: to compare the penetration capacity and the degree of microfiltration between a resinous sealant and an ionomer with and without ameloplasty, and with or without the placement of an adhesive prior to the placement of the resinous sealant. Methodology: 90 donated premolars, free of caries obtained previous indication orthodontic, later prophylaxis, were divided into six groups, in G1: ameloplasty was performed, acid etching, resinous sealant application, G2: ameloplasty, acid etching, adhesive application and sealant resinous, G3: acid etching, application of adhesive and resinous sealant, G4: application of ionomeric sealant, G5: acid etching, application of resinous sealant, G6: ameloplasty, application of ionomeric sealant. After thermocycling, the teeth were sectioned and the fragments observed under stereomicroscope and observation to the Scanning Electron Microscope. Resu...