The present study aims to obtain data that facilitate the design of an animal model that replicates salmonellosis produced by the serovar Typhimurium under experimental conditions by a single dose of streptomycin 50 mg orally 24 hours prior to the challenge with three concentrations of a virulent strain of Salmonella Typhimurium. Female guinea pigs (n=58) were used, distributed in 6 treatment groups of 8 animals each (3 groups to which streptomycin was administered prior to the challenge and 3 groups that were only challenged) and 2 control groups of 5 animals each (1 group treated with streptomycin and unchallenged and another untreated and unchallenged). The doses of the inoculum were 107, 108, 109/ml of S. Thiphimurium per group, respectively, and the animals were monitored for 30 days. Collective stool samples per group were collected to determine the excretion time of S. Typhimurium...