Objetivo: Evaluar “in vitro” la resistencia a la penetración bacteriana utilizando dos diferentes técnicas de obturación, compactación vertical y lateral con dos selladores endodónticos, uno a base de polidimetilsiloxano y el otro a base de hidróxido de calcio y resina epóxica frente a una cepa de Enterococcus faecalis ATCC 29212. Materiales y Métodos: Ochenta y cuatro dientes uniradiculares se dividieron de manera aleatoria en 4 grupos (n=20) y dos grupos de control (n= 4). El grupo I se obturó con la técnica de compactación lateral y sellador Sealer 26, el grupo II se obturó con la técnica de compactación vertical y sellador Sealer 26, el grupo III se obturó con la técnica de compactación lateral y sellador Roeko Seal y el grupo IV se obturó con la técnica de compactación vertical y sellador Roeko Seal. Se utilizó Enterococcus faecalis como marcador bacteriano ...

Objective: To determine the inhibitory effect of the methanolic fraction of propolis from Oxapampa, Peru, on different genotypes of Streptococcus mutans in children diagnosed with caries. Materials and methods: 150 oral swab samples were collected, seeded for isolation of S. mutans and then identified by biochemical tests of carbohydrate fermentation, Api 20 Strep (bioMérieux) and conventional PCR. The 138 strains isolated and identified from patients and the S. mutans ATCC® 25175™ control were confronted with the methanolic fraction by the disk diffusion method, having as positive control 0.12% chlorhexidine and negative control Milli-Q water and DMSO (1:1). Results: It was found that the inhibition diameter of the strains extracted from children diagnosed with caries against the methanolic fraction showed greater diameter (14.13 mm) in relation to the strain of S. mutans ATCC® 251...

Objective: To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material and Methods: Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the ...

Objective:To assess the antibacterial activity of Psidium guajavafractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalisin vitro. Material and Methods:Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajavawas evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobialsusceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey ...