We review the evolutionary sequence of laboratory technology in assisted reproduction. From the first attempts to manipulate preimplantational embryos recovered from animal oviducts in 1912; the direct transfers or with short incubation periods for receptor mothers; the complex biochemical process of preparation and supplementation of culture media from saline solutions chemically defined; the transition of this technology to humans basically in vitro oocyte maturation and selection of motile sperm; to proceedings ending with the first clinical application described in 1980.

We review the evolutionary sequence of laboratory technology in assisted reproduction. From the first attempts to manipulate preimplantational embryos recovered from animal oviducts in 1912; the direct transfers or with short incubation periods for receptor mothers; the complex biochemical process of preparation and supplementation of culture media from saline solutions chemically defined; the transition of this technology to humans basically in vitro oocyte maturation and selection of motile sperm; to proceedings ending with the first clinical application described in 1980.

En Perú, se realizaron los primeros procedimientos de reproducción humana asistida (RHA) en 1989 por el Grupo PRANOR. Veinticinco años más tarde, en 2014, la secuencia de desarrollo de esta tecnología se revisa, identificándose a nivel mundial los hitos principales del desarrollo. En este trabajo, dos períodos de desarrollo tecnológico de la RHA en Perú se fijan, el primero entre 1989 y 1998, y el segundo entre 1999 y 2014; definiéndose conceptos, técnicas y terminología específica para facilitar su comprensión.

In Peru, the first procedures of assisted human reproduction (AHR) were performed in 1989 by the PRANOR Group. Twenty-five years later, in 2014, the sequence of development of this technology is reviewed, identifying globally the top developmental milestones.In this paper, two periods of technological development of the RHA in Peru are set, the first between 1989 and 1998, and the second between 1999 and 2014; defining concepts, techniques and specific terminology for ease of understanding.

Using the primordial germ cells transplant technique, we could be able preserve and multiply pluripotent cells in the receptor for a long period of time. In this work, We aim to evaluate intraluminal colonization of a cellular gonocyte suspension from 14.5 dpc fetus. Cellular suspension with PGC's were isolated from fetus male mice by two enzymatic digestion steps, and cellular suspensions were transplanted into the rete testis of the receptor animals that were previously injected with Busulfan to decrease their own spermatogenesis. In this research the intraluminal colonization was identified in 13.27%, demonstrating that transplantation of a cellular suspension from gonocytes of fetus of 14.5 dpc containing PGCs can colonize the seminiferous tubules and support the spermatogenesis.

Using the primordial germ cells transplant technique, we could be able preserve and multiply pluripotent cells in the receptor for a long period of time. In this work, We aim to evaluate intraluminal colonization of a cellular gonocyte suspension from 14.5 dpc fetus. Cellular suspension with PGC's were isolated from fetus male mice by two enzymatic digestion steps, and cellular suspensions were transplanted into the rete testis of the receptor animals that were previously injected with Busulfan to decrease their own spermatogenesis. In this research the intraluminal colonization was identified in 13.27%, demonstrating that transplantation of a cellular suspension from gonocytes of fetus of 14.5 dpc containing PGCs can colonize the seminiferous tubules and support the spermatogenesis.