The objectives of this study were to establish the phenotypic antimicrobial sensitivity of the isolates from Peruvian rainbow trout using the procedures recommended by OIE

The purpose of this study was to characterize strains of Pasteurella multocida isolated from lungs of 1-2 months old alpacas with signs of pneumonia, collected in 2014. The BOX-PCR technique was used to demonstrate genetic diversity among strains. Twenty-four strains of P. multocida were isolated from 46 animals through biochemical identification tests. The BOX-PCR analysis showed two amplified band patterns, where 22 strains were grouped in one cluster and two strains in another cluster. The present study showed genetic homogeneity in most of the strains, evidencing a common source of infection that affected the animals.

Escherichia coli is associated with diarrhoea that can cause death in young alpacas. Patotypes of E. coli use the fimbriae to adhere to the host in the first stage of pathogenesis. Therefore, the present study aimed to develop and produce a recombinant protein of E. coli fimbrial adhesin F17 and to evaluate its immunogenicity in alpaca peripheral blood mononuclear cells (PBMC). BL21 was used as an expression vector, the recombinant protein was purified by immobilized affinity chromatography and the production of PBMC cytokines was evaluated 48 and 72 hours after the challenge with F17. The results indicate a significant increase in the production of Th1/Th2 cytokines, predominantly IFN-γ and IL-4. Therefore, the BL21-F17 mixture could be considered as a potential vaccine candidate for the prevention of diarrhoea in alpacas produced by E. coli.

The purpose of this study was to characterize strains of Pasteurella multocida isolated from lungs of 1-2 months old alpacas with signs of pneumonia, collected in 2014. The BOX-PCR technique was used to demonstrate genetic diversity among strains. Twenty-four strains of P. multocida were isolated from 46 animals through biochemical identification tests. The BOX-PCR analysis showed two amplified band patterns, where 22 strains were grouped in one cluster and two strains in another cluster. The present study showed genetic homogeneity in most of the strains, evidencing a common source of infection that affected the animals.

Escherichia coli is associated with diarrhoea that can cause death in young alpacas. Patotypes of E. coli use the fimbriae to adhere to the host in the first stage of pathogenesis. Therefore, the present study aimed to develop and produce a recombinant protein of E. coli fimbrial adhesin F17 and to evaluate its immunogenicity in alpaca peripheral blood mononuclear cells (PBMC). BL21 was used as an expression vector, the recombinant protein was purified by immobilized affinity chromatography and the production of PBMC cytokines was evaluated 48 and 72 hours after the challenge with F17. The results indicate a significant increase in the production of Th1/Th2 cytokines, predominantly IFN-γ and IL-4. Therefore, the BL21-F17 mixture could be considered as a potential vaccine candidate for the prevention of diarrhoea in alpacas produced by E. coli.

The aim of the study was to evaluate the in vitro immunogenic activity of a recombinant P6-like protein from a culture of Pasteurella multocida isolated from pneumonia cases in young alpacas. Blood peripheral mononuclear cells (PBMCs) were challenged with a recombinant P6-like protein at a concentration of 10 ng per sample, from 3 to 72 hours. Total RNA was extracted to perform the real-time RT-PCR test to observe cytokine expression levels of the Th1 immune response (TNF-α, IFN-γ and IL-2) and Th2 (IL-10 and IL-4) in the established times. Both Th1 and Th2 profile cytokines expressed a greater number of times than PBMC not exposed to the recombinant protein, where at 24 and 48 hours showed the greater expressions. Likewise, an apparent trend toward the Th2 profile was found, but at levels that did not influence the expression of cytokines in the other profile.

The aim of the study was to evaluate the in vitro immunogenic activity of a recombinant P6-like protein from a culture of Pasteurella multocida isolated from pneumonia cases in young alpacas. Blood peripheral mononuclear cells (PBMCs) were challenged with a recombinant P6-like protein at a concentration of 10 ng per sample, from 3 to 72 hours. Total RNA was extracted to perform the real-time RT-PCR test to observe cytokine expression levels of the Th1 immune response (TNF-α, IFN-γ and IL-2) and Th2 (IL-10 and IL-4) in the established times. Both Th1 and Th2 profile cytokines expressed a greater number of times than PBMC not exposed to the recombinant protein, where at 24 and 48 hours showed the greater expressions. Likewise, an apparent trend toward the Th2 profile was found, but at levels that did not influence the expression of cytokines in the other profile.

Trabajo presentado en el 8th International Symposium on Andean Geodynamics (ISAG), realizado en Quito-Ecuador, del 24-26 setiembre, 2019. Evento organizado por el Instituto Geofísico, Escuela Politécnica Nacional (IG-EPN) del Ecuador, y el French Institut de Recherche pour le Développement (IRD).