The aim of the present study was to relate the holistic performance of teachers to the overall development of fifth-grade students at Colegio Cooperativo César Vallejo Iquitos in 2023. The methodology employed was based on a relational approach, with a cross-sectional and bivariate field design. A population and sample of 271 students were surveyed using validated questionnaires. The correlation results indicated a significant relationship between the holistic performance of teachers and the overall development of fifth-grade students at Colegio Cooperativo César Vallejo Iquitos in 2023. The obtained p-value was lower than the significance level (α = .05), leading to the rejection of the null hypothesis and acceptance of the alternative hypothesis. Additionally, the correlation coefficient (rho = .414) revealed a moderately positive relationship between both variables. In conclusion, ...

This work was supported by a grant from CONCYTEC. We are very grateful to Holger Valqui and Oscar Moran for their critical comments, and to ClaudiaMachicado and Jose Choufor their comments and help in compiling and analysing part of the DNA sequences that support this work. Thanks also to Arman- do Bernui, Javier Espinoza, Jairzhinho Ramos, Luis Marky and Cristian Orrego, for their contribution to discussions, and especially to Mrs Ellen M. Pragen for her editorial work.

Recent publications have shown that active RNA polymerase (RNAP) from Mycobacterium tuberculosis (MtbRNAP) can be produced by expressing all four subunits in a single recombinant Escherichia coli strain [1–3]. By reducing the number of plasmids and changing the codon usage of the Mtb genes in the co-expression system published by Banerjee et al. [1], we present a simplified, detailed and reproducible protocol for the purification of recombinant MtbRNAP containing the ω subunit. Moreover, we describe the formation of ternary elongation complexes (TECs) with a short fluorescence-labeled RNA primer and DNA oligonucleotides, suitable for transcription elongation studies.

This work was supported by Fondecyt 11110534 (to M.B.), 1151274 (to M.B.), 11130263 (to C.A.M.W.), Anillo 1107 (to M.B.), and Chile and Fondecyt 196–2013 (to D.G.G.) CONCYTEC, Perú. This research was partially supported by the supercomputing infrastructure of the NLHPC (ECM-02) at Universidad de Chile. A.B. was supported by Conicyt master fellowship 22121199. We gratefully acknowledge Professor Alexander Vologodskii from New York University for helpful comments and advice. Travel for C.B. to and from Chile and Peru was also partially supported by the Howard Hughes Medical Institute, NIH grant R01GM032543, and the U.S. Department of Energy Office of Basic Energy Sciences Nanomachine Program under contract no. DE-AC02-05CH11231.