Objetivo: Determinar la sensibilidad de la PCR digital para detectar mutaciones en el gen PIK3CA en tumor y su nivel de concordancia en el plasma de pacientes peruanas con cáncer de mama. Metodología: Estudio observacional, analítico, transversal, en el que se recolectaron 59 muestras de tumor y 43 de plasma preoperatorio de pacientes con cáncer de mama. Los tumores fueron analizados por secuenciación Sanger y PCR digital para tres mutaciones comunes de PIK3CA (E545K, H1047R y H1047L), mientras que el plasma fue analizado solo por PCR digital para las dos primeras mutaciones. Resultados: La secuenciación de los tumores identificó en total siete pacientes (11.8%) con mutaciones en el gen PIK3CA, de las cuales tres corresponden a la mutación H1047R y cuatro a E545K. La PCR digital identificó las mismas, pero detectó 37 pacientes adicionales con por lo menos una mutación, 33 de E...

Background: Promoter hypermethylation is one of the enabling mechanisms of hallmarks of cancer. Tumor suppressor genes like RARB and GSTP1 have been reported as hypermethylated in breast cancer tumors compared with normal tissues in several populations. This case–control study aimed to determine the association between the promoter methylation ratio (PMR) of RARB and GSTP1 genes (separately and as a group) with breast cancer and its clinical-pathological variables in Peruvian patients, using a liquid biopsy approach. Methods: A total of 58 breast cancer patients and 58 healthy controls, matched by age, participated in the study. We exacted cell-free DNA (cfDNA) from blood plasma and converted it by bisulfite salts. Methylight PCR was performed to obtain the PMR value of the studied genes. We determined the association between PMR and breast cancer, in addition to other clinicopathologi...

Objective: To determine the characteristics of the place of residence and the cardiovascular risk factors in a Peruvian jungle population in July 2014. Materials and methods: A descriptive cross-sectional study conducted in individuals between 30 and 74 years old without a diagnosis of or treatment for a cardiovascular disease in an urban and rural population of the district of Yantaló. The sample was obtained using the Power Analysis and Sample Size Software (PASS) program, and consisted of 268 people (152 urban and 116 rural residents). The studied factors were hypertension (HBP), diabetes mellitus (DM), body mass index (BMI), waist-hip ratio (WHR) and smoking. Results: The urban population had higher rates of hypertension (18.4%), while the other risk factors showed no significant differences between the two populations. Conclusions: The only risk factor that showed a significant ass...