Aiming to determine the genetic variability of accessions of Chenopodium quinoa Willd. "Quinoa" from the Germplasm Bank of INIA - Ayacucho, analyze 29 accessions, which were characterized using AFLP molecular markers. The quality of the DNA extracted by the Doyle and modified Doyle method was optimal for all samples, obtaining modifications in a range of 100 - 400 ng / μl. In the standardization of the AFLP technique, it was determined that the proteins between 100 and 200 ng / μl of digested DNA were adequate to efficiently develop the AFLP technique, and it was also determined that the preamplification products that allowed the resolution amplification profiles variables were higher with a 1:4 ratio. A total of 7 primer combinations generated 220 DNA bands, being 201 polymorphic loci. Molecular data grouped the accessions into seven groups at a similarity of 0.81 ultrametric units, o...

Aiming to determine the genetic variability of accessions of Chenopodium quinoa Willd. "Quinoa" from the Germplasm Bank of INIA - Ayacucho, analyze 29 accessions, which were characterized using AFLP molecular markers. The quality of the DNA extracted by the Doyle and modified Doyle method was optimal for all samples, obtaining modifications in a range of 100 - 400 ng / μl. In the standardization of the AFLP technique, it was determined that the proteins between 100 and 200 ng / μl of digested DNA were adequate to efficiently develop the AFLP technique, and it was also determined that the preamplification products that allowed the resolution amplification profiles variables were higher with a 1:4 ratio. A total of 7 primer combinations generated 220 DNA bands, being 201 polymorphic loci. Molecular data grouped the accessions into seven groups at a similarity of 0.81 ultrametric units, o...

La enfermedad denominada marchitamiento vascular es un problema que produce perdidas en la producción de los cultivos de Caesalpinia spinosa “tara”. El objetivo de la investigación fue identificar el agente causal del marchitamiento y determinar el efecto antagónico de cepas de Bacillus spp. y Trichoderma sp. Fueron muestreadas plantas de tara con síntomas de la enfermedad y desde el suelo. Se aislaron 27 cepas de hongos filamentosos que fueron identificados mediante el método de Pitt basado en las claves taxonómicas. El efecto antagónico se realizó mediante pareamientos in vitro en medio Agar Papa Dextrosa, enfrentando a cepas de Fusarium spp. con dos cepas de Trichoderma sp. y 18 de Bacillus spp. como potenciales antagonistas. Los resultados constataron que las cepas de Fusarium spp., son las causantes del marchitamiento vascular progresivo en tara. La evaluación del antag...