The fique crop has great potential for the development of the natural fiber market as a beneficial alternative for the protection of the environment. In Colombia, one of the main limitations of fiber production in fique plants is the viral disease Macana caused by Furcraea Necrotic Streak Virus (FNSV). This work aimed to validate the detection of FNSV in asexual planting material from one of the main producing areas and thus contribute to preventing the spread of the disease in the country. The analysis of plants from different geographic altitudes in Cauca, Colombia, showed a positive correlation with the prevalence of Macana disease (being more significant at higher altitudes) but not with the severity of the symptoms. The detection of FNSV on seeds by dot blot immunobinding assay (DBIA) using a polyclonal antibody IgY showed sensitivity (79 %) and specificity (80 %) when sprouts were ...