Introduction. Hydrogen sulfide (H2S) is toxic and lethal to mammals at high concentrations, but at low concentrations may be beneficial. Objective. Assessment of hydrogen sulfide harmlessness on Cavia porcellus (guinea pigs) cardiac tissue in vivo. Design. Experimental study. Setting. School of Medicine Pharmacology Laboratory, Universidad Nacional Mayor de San Marcos, Lima, Peru, and Experimental Surgery Unit at Instituto Nacional de Salud del Niño, Lima, Peru. Biological material: Twenty one Cavia porcellus (guinea pigs). Interventions. The experimental design considered three groups: 1) Clean air (control); 2) H2S at 80 ± 20 ppm (experimental, gaseous exposure); 3) Sevoflurane 1.5% (gold standard). Animals were placed in a hood where they were nebulized for twenty minutes; at the end of the exposure, the heart was withdrawn for histopathological study. Main outcome measures. Cardiac...

Evalúa el efecto modulador del H2S a 80 ppm sobre el metabolismo basal en Cavia porcellus (cuy) normales. Para ello mide el consumo de oxígeno y producción de dióxido de carbono del Cavia porcellus (cuy) normales inmediatamente antes y después de la exposición al sulfuro de hidrógeno. Para luego determinar los cambios histológicos a nivel de tejido pulmonar, cardiaco y renal, y los niveles de hemoglobina entre los grupos experimentales (control, experimental y gold estándar).

Introduction. Antioxidants have shown chemopreventive potential in degenerative, inflammatory, autoimmune, oncology and respiratory distress associated pathologies. Objective. To assess the phytochemical and antioxidant properties in vitro by DPPH and ABTS method. Design. Observational analytical. Location. Laboratory of Experimental Pharmacology, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological/Chemical Material. Aloe vera leaves, Plukenetia volubilis seeds, Caiophora carduifolia leaves-stalks, Cecropia membranacea leaves. Interventions. Observation and analysis of the antioxidant capacity by DPPH-method mean effective concentration (EC50) of the extracts and trolox equivalent antioxidant capacity by the ABTS method. Main outcome measures. Phytochemical preliminary march, antioxidant percent uptake inhibition by DPPH radical, determination of equival...