“Potential use of organic waste and microalgae generates bioelectricity and thereby reduces harmful effects on the environment. These residues are used due to their high content of electron-generating microorganisms. However, so far, they have not been used simultaneously. Therefore, this research uses mango waste and microalgae Spirulina sp. in double-chamber microbial fuel cells to generate bioelectricity. The cells were made at a laboratory scale using zinc and copper electrodes, achieving a maximum current and voltage of 7.5948 ± 0.3109 mA and 0.84546 ± 0.314 V, with maximum electrical conductivity of the substrate being 157.712 ± 4.56 mS/cm and an optimum operating pH being 5.016 ± 0.086. The cells showed a low internal resistance of approximately 205.056 ± 25 Ω, and a maximum power density of 657.958 ± 21.114 mW/cm2 at a current density of 4.484 A/cm2 . This research provi...