Background: Stingless bees (Apidae: Meliponini) are critical pollinators in the Peruvian Amazon, sustaining biodiversity, crop productivity, and indigenous livelihoods. Despite their ecological and economic importance, the effects of deforestation and illegal logging on their populations remain poorly understood. Nesting tree loss, habitat fragmentation, and changes in elevation influence stingless bee distribution, yet conservation efforts remain insufficient due to a lack of spatial data on their vulnerability. Results: This study examines the spatial distribution, elevation trends, and deforestation threats affecting Melipona eburnea and Tetragonisca angustula, with a focus on tree nesting preferences in the Biosphere Reserve Avireri-Vraem. Using literature reviews, field observations, and GPS surveys, we assessed species distribution patterns, deforestation risks, and nesting tree as...