Fusarium oxysporum f. sp. cubense (Foc) poses a significant threat to global banana production. This systematic review updates current knowledge on the efficacy of various antagonistic microorganisms in controlling Foc, considering the recent spread of this disease to new regions. The studies were systematically analyzed, focusing on methodologies, results, and conclusions to provide a comprehensive overview of current research and its practical implications. A total of 118 studies were reviewed, covering the use of antagonistic microorganisms such as Trichoderma spp., Bacillus spp., Streptomyces spp., and Pseudomonas spp., both in pure cultures and in consortia. Most studies focused on controlling Foc TR4 in Cavendish subgroup bananas and originated from Asia. Microbial consortia demonstrated a higher control percentage with lower variability, particularly in genera such as Pseudomonas....