The follicular dynamics was evaluated by ultrasonography in Brown Swiss cattle subjected to a protocol of synchronization of estrus with progestogens in the Peruvian highlands. Three groups of Brown Swiss cattle of nine females each were evaluated. The groups were: lactating cows (<120 and >260 days in milk) and heifers. For synchronization, on day 0 a progesterone-based intravaginal device was applied, and 2 mg estradiol benzoate was injected, on day 8 the device was removed and 0.15 mg cloprostenol and 400 IU eCG were applied, and on day 10 1.05 mg GnRH was applied. Artificial insemination at fixed time was done 12 h after the application of GnRH. The ultrasound evaluation was done on days 8 and 10 to determine the growth rate and maximum diameter of the dominant follicle in the three groups. The follicular growth rate was 2.3 ± 1.5, 2.4 ± 1.8 and 2.3 ± 1.8 mm/day for the grou...

The aim of this study was to determine the effect of three freezing curves on the post-thaw viability of spermatozoa collected from the vas deferens of llamas. Six male llamas with surgical diversion of the vas deferens were used. The samples of the six males were mixed for processing (pools) and diluted with Tris-egg yolk. Cooling was carried out to 5 °C where the dilution was completed (dilutor with glycerol) and was maintained for 30 minutes (equilibrium phase). Samples in 0.25 ml straws were subjected to freezing using a temperature drop rate of -20°C/min until reaching -80°C (TI), -100°C (TII) and -120°C (TIII) in 4, 5 and 6 minutes, respectively, to finally store them in liquid nitrogen. The samples were collected for three months (n=19 pools). Total motility (progressive, circular, oscillatory motility), viability and membrane functionality were determined after collection, i...

The follicular dynamics was evaluated by ultrasonography in Brown Swiss cattle subjected to a protocol of synchronization of estrus with progestogens in the Peruvian highlands. Three groups of Brown Swiss cattle of nine females each were evaluated. The groups were: lactating cows (<120 and >260 days in milk) and heifers. For synchronization, on day 0 a progesterone-based intravaginal device was applied, and 2 mg estradiol benzoate was injected, on day 8 the device was removed and 0.15 mg cloprostenol and 400 IU eCG were applied, and on day 10 1.05 mg GnRH was applied. Artificial insemination at fixed time was done 12 h after the application of GnRH. The ultrasound evaluation was done on days 8 and 10 to determine the growth rate and maximum diameter of the dominant follicle in the three groups. The follicular growth rate was 2.3 ± 1.5, 2.4 ± 1.8 and 2.3 ± 1.8 mm/day for the grou...