In the present work, the roots of Colignonia parviflora subsp. biumbellata, was acquired in the city of Chinchero at 3780 msnm. Twenty four chemical compounds were identified among methyl esters, monoterpenes, sesquiterpenes, phenylpropanoid and aliphatic alcohols in the root essential oil, the identification was through the comparasion of the mass spectra with the data bank of the CG-MS system (NIST 14) and by the analysis of their mass spectra obtained by gas chromatography coupled to a mass spectrometer. In the apolar phase of the root juice, its major fatty acids and secondary metabolites β-sitosterol, stigmasterol, stigmast- 7-en-3β-ol, stigmast-7,22-dien-3β-ol, methyl ester of ferulic acid and oleanic acid.