Background:Dionaea muscipula called "venusflytrap" isthe only species ofthe genusDionaea belonging to the group of "carnivorous plants" that besides its ornamental appeal nowadays is studied for the isolation of secondary metabolites, having finding various compounds with direct activity in different diseases. Plumbagin (2-methyl-5-hydroxy-1,4-naphthoquinone), has been reported in different genressuch as Plumbago species(Plumbaginaceae), Drosera and Dionaea (Droseraceae) and Diospyros (Ebenaceae) describing activities like antispasmodic, antibacterial, antiparasitic antifungal, anti-rheumatic pain and anti-cancer. The aim of this study is to develop a method by HPLC UV reliable for the determination of plumbagina. The method presented a linearity r 2 = 0.997569 for concentrations between 10 and 50 ug / ml, with limits of detection (LOD) and quantitation (LOQ) of 0.09 g / ml and 0.3 ug/ m...

El Perú vive un entorno favorable de crecimiento en su economía desde los últimos 20 años a pesar de su desaceleración en los 03 últimos años, estimándose que el crecimiento continúe en los próximos años. El sector servicios, donde se ubica el rubro de restaurantes, tiene un gran aporte en el crecimiento económico del PBI y mantiene incrementos anuales mayores al 6%. Todo ello favorecido por el importante desarrollo de nuestra gastronomía, que en los últimos años ha logrado un amplio reconocimiento en el mundo y que incentiva al consumo interno de la población así como la oferta en la apertura de restaurantes. Por otro lado, el crecimiento de la infraestructura y la tecnología de información vienen posibilitando el desarrollo de nuevos tipos de negocio y marketing, existiendo aplicaciones para teléfonos móviles que permite a los usuarios llamar a taxis, descargar pel...

Brown algae are a source of high content of polysaccharides such as fucoidans that have important immunomodulatory properties. The aim was to determine the viability of human peripheral blood mononuclear cells (hPBMC), production of nitric oxide (NO), reactive oxygen species (ROS) and the proinflammatory cytokines IL-1α, IL-6, TNF-α and IFN -γ in cultures treated with fucoidan from Lessonia trabeculata. Fucoidan from Lessonia trabeculata from San Nicolás de Marcona-Ica Bay was used. The hPBMC were isolated using Ficoll-Hypaque, distributed at a concentration of 1x105 cells/well in complete RPMI-1640 medium and treated with different concentrations of fucoidan for 24 and 48 h. The cytotoxic activity was determined by the reduction of MTT, NO production by the Griess reaction and ROS by the reduction of NBT. The production of cytokines was quantified by ELISA. The fucoidan of L. trabec...

Brown algae are a source of high content of polysaccharides such as fucoidans that have important immunomodulatory properties. The aim was to determine the viability of human peripheral blood mononuclear cells (hPBMC), production of nitric oxide (NO), reactive oxygen species (ROS) and the proinflammatory cytokines IL-1α, IL-6, TNF-α and IFN -γ in cultures treated with fucoidan from Lessonia trabeculata. Fucoidan from Lessonia trabeculata from San Nicolás de Marcona-Ica Bay was used. The hPBMC were isolated using Ficoll-Hypaque, distributed at a concentration of 1x105 cells/well in complete RPMI-1640 medium and treated with different concentrations of fucoidan for 24 and 48 h. The cytotoxic activity was determined by the reduction of MTT, NO production by the Griess reaction and ROS by the reduction of NBT. The production of cytokines was quantified by ELISA. The fucoidan of L. trabec...

Reactive oxygen species (ROS) are normally produced during cell metabolism, there is strong evidence to suggest that ROS produced in excess impair the cell and may be etiologically related to various neurodegenerative diseases. This study was undertaken to examine the effects of Lepidium meyenii (MACA) methanol leaf extract on neurotoxicity in PC12 cell exposed to 6-hydroxydopamine (6-OHDA). Fresh samples of “maca” leaves were processed in order to obtain foliar extracts and to evaluate the neurobiological activity on PC12 cells, subjected to the cytotoxic effect of 6-OHDA through the determination of the capacity antioxidant, cell viability and cytotoxicity assays on PC12 cells. The results of the tests of antioxidant activity, showed maximum values of 2262.37 and 1305.36 expressed in Trolox equivalents (TEAC), for the methanolic and aqueous fractions respectively. Cell viability as...