Introduction: Human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) infections are a global public health concern. In 2019, there were 295.9 million people with chronic hepatitis B and 57.8 million with HCV, and less than 20% were aware of their status. In 2017, there were 37 million people with HIV, and 75% were diagnosed. Creating accessible screening strategies remains a challenge. The use of rapid diagnostic tests (RDTs) is a cost-effective and simple option. The endoscopy unit is an ideal setting due to its high patient flow and qualified personnel. Objective: To evaluate the use and acceptance of two RDTs for detecting HIV, HBV, and HCV in patients undergoing endoscopic examination and to determine the percentage of refusal to undergo testing. Materials and methods: An observational cross-sectional pilot study was conducted. Adults enterin...