The aim of this study was to analyse the gene and genotypic parameters using microsatellites of Creole bovine (CB) from the high southern tropics of Ecuador and contrasting them with registered Holstein bovine (HB); For this, blood was collected from 7 CC and 3 BC subpopulations. Fifteen markers grouped into 4 mixtures were analysed; 1) CSRM60, INRA083, CSSM66; 2) ETH3, HEL9, TGLA53, BM1818, ILSTS006; 3) BM2113, ETH225, TGLA122, ETH10; and 4) TGLA227, INRA032, SPS115. Genetic variation parameters were determined: number of alleles (Na), number of effective alleles (Ne), frequency of null alleles (f-Null), observed heterozygosity (Ho) and expected (He), Polymorphism Information Content (PIC), inbreeding coefficients (Fis), Hardy-Weinberg equilibrium (HWe). In addition, a dendrogram was constructed based on Nei’s distances and identities, and finally, the AMOVA multilocus analysis. In mo...