Evaluación de proteínas recombinantes del estadío asexual de Plasmodium spp. como nuevos marcadores de seroprevalencia de malaria en la Amazonía peruana

• Autor: Garro Gamarra, Katherine Daniela
• Formato: tesis de maestría
• Fecha de Publicación: 2019
• Institución: Universidad Peruana Cayetano Heredia
• Repositorio: UPCH-Institucional
• Lenguaje: español
• OAI Identifier: oai:repositorio.upch.edu.pe:20.500.12866/9055
• Enlace del recurso: https://hdl.handle.net/20.500.12866/9055
• Nivel de acceso: acceso abierto
• Materia: Malaria; Plasmodium vivax; Plasmodium falciparum; Baculovirus; Seromarcadores; Amazonía Peruana; PvMSP8; PvMSP10; PfMSP10; PfRH2B; https://purl.org/pe-repo/ocde/ford#1.06.03; https://purl.org/pe-repo/ocde/ford#3.03.06; https://purl.org/pe-repo/ocde/ford#3.03.07; https://purl.org/pe-repo/ocde/ford#3.03.08

Malaria seroprevalence evaluation is a transcendental step to carry out real monitoring of disease transmission. For this reason, this study have focused on the production (Baculovirus expression and insect cells) and evaluation of the recombinant rPvMSP8 and rPfRH2B proteins of Plasmodium vivax and P. falciparum respectively, as new malaria sero-marker in the Peruvian Amazon. These proteins were evaluated together with rPvMSP10 and rPfMSP10 proteins (previously evaluated by our group) in conjunction with reference seromarchers (rPvMSP1, rPvAMA1, rPfMSP1 and rPfGLURP). For this, 421 serum samples from a cross sectional study population of two Loreto communities, Cahuide (n = 257) and Lupuna (n = 164), were used to assess specific IgG levels against the 8 recombinant proteins using a Luminex® multiplex assay. A multivariate regression analysis indicated that the IgG titers for the 4 novels proteins were associated with the patients' age, showing a possible cumulative response, generating humoral memory, both in exposed and unexposed patients. In addition, IgG titers for PvMSP10 and PvMSP8 were significantly associated with the history of P. vivax passed infections only in the low transmission community (Cahuide). These results suggest that in order to maintain and detect specific circulating levels of IgG against these proteins, recurrent exposure to Plasmodium spp. is necessary, and thus would be suitable as possible serological markers useful for the identification of remaining transmission hotspots. However, additional studies are necessary to confirm these proteins as seromarkers.