Antioxidant activity of Origanum vulgare extract gel. Is it important for oral health?. Preliminary study

• Autores: Galvez-Calla, Luis, Alvarez-Páucar, María A., Alcázar-Aguilar, Omar, Mayta-Tovalino, Frank, Lozano-Castro, Felipe, Cordova-Huayanay, Edwin, Revoredo-Morote, Roxana
• Formato: artículo
• Fecha de Publicación: 2021
• Institución: Universidad Peruana Cayetano Heredia
• Repositorio: Revistas - Universidad Peruana Cayetano Heredia
• Lenguaje: español
• OAI Identifier: oai:revistas.upch.edu.pe:article/3921
• Enlace del recurso: https://revistas.upch.edu.pe/index.php/REH/article/view/3921
• Nivel de acceso: acceso abierto
• Materia: antioxidantes; estrés oxidativo; Origanum vulgare; radicales libres; salud bucal; antioxidants; free radicals; oral health; oxidative stress

Objective: To determine the antioxidant activity of the hydroalcoholic extract of Origanum vulgare through DPPH, ABTS and FRAP essays. Material and Methods: The sample collected from Origanum vulgare was dried, reduced in size and placed to macerate 500 grams of dry sample in 1000 mL of 97% ethanol for one week. After time, the macerate was processed through a filter and concentrated in an oven. The formulations were carried out and the dilutions were analyzed with the antioxidant essays. Results: DPPH Method, the 25% gel showed an IC50 of 98.485 mg / mL equivalent to the dilution of 78.789% and for the Trolox® standard, it presented IC50 of 2.48 µg / mL. ABTS Method, the 25% gel formulation presented IC50 of 3.687 mg / mL equivalent to a dilution of 77.75% and for the Trolox® standard it presented IC50 of 2.99 µg / mL, a difference from the other formulations. The results evidenced a relation between the percentage of inhibition and concentration of the samples with an accepted correlation (R2) for the gels at 25%, 50%, 75% of extract of Origanum vulgare and Trolox® of 0.9972; 0.9987 and 0.9986 respectively. FRAP Method, with fast action during the 4 minutes, being 125 mg of extract contained in the gel 25% equivalent to 4mg of Trolox®. Conclusions: It was determined that the antioxidant activity equivalent to Trolox®, with antioxidant assays, with the best average free radical uptake power (Trolox / mg) of extract was present in the gel of Extract of Origanum vulgare 25%.