Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria

Descripción del Articulo

The characteristics of a strain of Bacillus amyloliquefaciens isolated from the intestine of Arapaima gigas, selected for its ability to generate inhibition of multiple pathogenic bacteria of fishes were analyzed by using molecular tools such as PCR and mass spectrometry - MALDI TOF / TOFM. The resu...

Descripción completa

Detalles Bibliográficos
Autores: Feria Zevallos, Manuel Alberto, Castañeda, Arnaldo, Toledo, Odalis, Caballero, Deysy, Cueva, Mario, Cedeño, Virna
Formato: artículo
Fecha de Publicación:2019
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/15407
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/15407
Nivel de acceso:acceso abierto
Materia:Bacillus amyloliquefaciens
MALDI TOF
probiotic
Arapaima gigas
probióticos
id REVUNMSM_f279aa2f1d3456cde34cb792fbcdb007
oai_identifier_str oai:ojs.csi.unmsm:article/15407
network_acronym_str REVUNMSM
network_name_str Revistas - Universidad Nacional Mayor de San Marcos
repository_id_str
spelling Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteriaCaracterización molecular ómica de una cepa de Bacillus amyloliquefaciens aislada de la microbiota del paiche Arapaima gigas con actividad antagonista contra bacterias patógenas de pecesFeria Zevallos, Manuel AlbertoCastañeda, ArnaldoToledo, OdalisCaballero, DeysyCueva, MarioCedeño, VirnaBacillus amyloliquefaciensMALDI TOFprobioticArapaima gigasBacillus amyloliquefaciensMALDI TOFprobióticosArapaima gigasThe characteristics of a strain of Bacillus amyloliquefaciens isolated from the intestine of Arapaima gigas, selected for its ability to generate inhibition of multiple pathogenic bacteria of fishes were analyzed by using molecular tools such as PCR and mass spectrometry - MALDI TOF / TOFM. The results showed through PCR that this strain has key genes for the generation of antimicrobial peptides such as bmyB (bacillomycin L synthetase B), fenD (fengycin synthetase), srfAA (subunit 1 surfactin synthetase), bacA (bacilysin biosynthesis protein) and iturin (iturin A). In addition, by mass spectrometry analysis were detected bacteriocins (plipastatin, gramicidin, fengycin, surfactin), intestine binding proteins (like-enolase), antimicrobial peptide transport proteins (ABC-transporters) and immune system stimulation proteins like flagelin. Collagenase, chitinases and xylose-isomerases proteins were also detected that contribute to the digestion and assimilation process. All these results allow to consider the multiple benefits of this strain to be used as a probiotic in fish culture.Se analizaron las características de una cepa de Bacillus amyloliquefaciens aislada del intestino de Arapaima gigas, seleccionada por su capacidad para generar inhibición de múltiples bacterias patógenas de peces, mediante el uso de herramientas moleculares como PCR y espectrometría de masas - MALDI TOF/TOF. Los resultados mostraron a través de PCR que esta cepa cuenta con genes claves para la generación de péptidos antimicrobianos como bmyB (bacillomycin L synthetase B), fenD (fengycin sintetasa), srfAA (subunidad 1 surfactin sintetasa), bacA (proteína de biosíntesis de bacilysin) e iturin (iturin A). Además, mediante el análisis por espectrometría de masas se detectaron bacteriocinas (plipastatin, gramicidin, fengycin, surfactin), proteínas de fijación al intestino (like-enolase), proteinas transportadoras de péptidos antimicrobianos (ABC-transporters) y proteínas de estimulación del sistema inmunitario como flagelin. También se detectaron proteínas tipo colagenasas, chitinasas e xilosa-isomerasas que contribuyen con el proceso de digestión y asimilación. Todos estos resultados permiten considerar los múltiples beneficios de esta cepa para ser utilizada como probiótico en el cultivo de peces.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2019-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1540710.15381/rivep.v30i2.15407Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 2 (2019); 908-922Revista de Investigaciones Veterinarias del Perú; Vol. 30 No. 2 (2019); 908-9221682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/15407/14092Derechos de autor 2019 Manuel Alberto Feria Zevalloshttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/154072019-07-05T17:17:55Z
dc.title.none.fl_str_mv Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
Caracterización molecular ómica de una cepa de Bacillus amyloliquefaciens aislada de la microbiota del paiche Arapaima gigas con actividad antagonista contra bacterias patógenas de peces
title Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
spellingShingle Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
Feria Zevallos, Manuel Alberto
Bacillus amyloliquefaciens
MALDI TOF
probiotic
Arapaima gigas
Bacillus amyloliquefaciens
MALDI TOF
probióticos
Arapaima gigas
title_short Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
title_full Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
title_fullStr Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
title_full_unstemmed Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
title_sort Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
dc.creator.none.fl_str_mv Feria Zevallos, Manuel Alberto
Castañeda, Arnaldo
Toledo, Odalis
Caballero, Deysy
Cueva, Mario
Cedeño, Virna
author Feria Zevallos, Manuel Alberto
author_facet Feria Zevallos, Manuel Alberto
Castañeda, Arnaldo
Toledo, Odalis
Caballero, Deysy
Cueva, Mario
Cedeño, Virna
author_role author
author2 Castañeda, Arnaldo
Toledo, Odalis
Caballero, Deysy
Cueva, Mario
Cedeño, Virna
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacillus amyloliquefaciens
MALDI TOF
probiotic
Arapaima gigas
Bacillus amyloliquefaciens
MALDI TOF
probióticos
Arapaima gigas
topic Bacillus amyloliquefaciens
MALDI TOF
probiotic
Arapaima gigas
Bacillus amyloliquefaciens
MALDI TOF
probióticos
Arapaima gigas
description The characteristics of a strain of Bacillus amyloliquefaciens isolated from the intestine of Arapaima gigas, selected for its ability to generate inhibition of multiple pathogenic bacteria of fishes were analyzed by using molecular tools such as PCR and mass spectrometry - MALDI TOF / TOFM. The results showed through PCR that this strain has key genes for the generation of antimicrobial peptides such as bmyB (bacillomycin L synthetase B), fenD (fengycin synthetase), srfAA (subunit 1 surfactin synthetase), bacA (bacilysin biosynthesis protein) and iturin (iturin A). In addition, by mass spectrometry analysis were detected bacteriocins (plipastatin, gramicidin, fengycin, surfactin), intestine binding proteins (like-enolase), antimicrobial peptide transport proteins (ABC-transporters) and immune system stimulation proteins like flagelin. Collagenase, chitinases and xylose-isomerases proteins were also detected that contribute to the digestion and assimilation process. All these results allow to consider the multiple benefits of this strain to be used as a probiotic in fish culture.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/15407
10.15381/rivep.v30i2.15407
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/15407
identifier_str_mv 10.15381/rivep.v30i2.15407
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/15407/14092
dc.rights.none.fl_str_mv Derechos de autor 2019 Manuel Alberto Feria Zevallos
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2019 Manuel Alberto Feria Zevallos
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 2 (2019); 908-922
Revista de Investigaciones Veterinarias del Perú; Vol. 30 No. 2 (2019); 908-922
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238230580264960
score 13.880932
Omic molecular characterization of a Bacillus amyloliquefaciens strain isolated from the paiche Arapaima gigas microbiota with antagonistic activity against fish pathogenic bacteria
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

Ejemplares Similares