IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER

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The aim of this study was to isolate and identify bacteria of Lactobacillus genus from poultry litter with 1, 2 or 3 uses and low ammonia records. Resistance for conditions at pH 5 and 8 was evaluated as well as the ability to inhibit activity in urease bacteria (Staphylococcus spp and Pseudomona sp...

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Detalles Bibliográficos
Autores: Shiva, Carlos, Jara, Luis M.
Formato: artículo
Fecha de Publicación:2013
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/2579
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2579
Nivel de acceso:acceso abierto
Materia:Lactobacillus
pollos de engorde
probióticos
aislamiento
amoníaco
broiler
probiotics
isolation
ammonia
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spelling IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTERIDENTIFICACIÓN Y CARACTERIZACIÓN MOLECULAR DE CEPAS NATIVAS DE LACTOBACILLUS AISLADAS DE MATERIAL DE CAMA DE POLLOS DE ENGORDEShiva, CarlosJara, Luis M.Lactobacilluspollos de engordeprobióticosaislamientoamoníacoLactobacillusbroilerprobioticsisolationammoniaThe aim of this study was to isolate and identify bacteria of Lactobacillus genus from poultry litter with 1, 2 or 3 uses and low ammonia records. Resistance for conditions at pH 5 and 8 was evaluated as well as the ability to inhibit activity in urease bacteria (Staphylococcus spp and Pseudomona spp) isolated from poultry litter. Seventy three suspected colonies of Lactobacillus were identified by culture in selective media and morphological patterns. Gram staining and catalase test revealed that only 19 strains were of Lactobacillus genus. Furthermore, through PCR was found that 53% corresponded to L. reuteri, 32% to L. plantarum, 10% to L. vaginalis and 5% to L. salivarius. All isolates survived at conditions of pH 5 and 8 and all strains produced some type of active biocin against Staphylococci spp or Pseudomona spp which might be useful as additive to regulate the microbiota in poultry litter.El objetivo del presente estudio fue aislar e identificar bacterias del género Lactobacillus provenientes de material de cama de 1 a 3 usos con bajos registros de amoniaco de granjas de pollos de engorde. Se evaluó la resistencia a condiciones de pH 5 y 8, así como la capacidad de inhibición sobre bacterias con actividad ureasa (Staphylococcus spp y Pseudomona spp) aisladas a partir de material de cama. Se aislaron 73 colonias consideradas como sospechosas de Lactobacillus a partir de siembra en medios selectivos y patrones morfológicos. Mediante tinción de Gram y prueba de catalasa se comprobó que 19 cepas correspondieron al género Lactobacillus y con PCR se encontró que el 53% correspondió a L. reuteri, el 32% a L. plantarum, el 10% a L. vaginalis y el 5% a L. salivarius. Todas las cepas sobrevivieron a condiciones de pH 5 y 8 y todas produjeron algún tipo de biocina activa contra Staphylococcus spp o Pseudomonas spp que podrían ser útiles a futuro como aditivo para regular la microbiota en el material de cama.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2013-09-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/257910.15381/rivep.v24i3.2579Revista de Investigaciones Veterinarias del Perú; Vol. 24 Núm. 3 (2013); 307-315Revista de Investigaciones Veterinarias del Perú; Vol. 24 No. 3 (2013); 307-3151682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2579/2256Derechos de autor 2013 Carlos Shiva, Luis M. Jarahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/25792020-03-30T19:41:02Z
dc.title.none.fl_str_mv IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
IDENTIFICACIÓN Y CARACTERIZACIÓN MOLECULAR DE CEPAS NATIVAS DE LACTOBACILLUS AISLADAS DE MATERIAL DE CAMA DE POLLOS DE ENGORDE
title IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
spellingShingle IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
Shiva, Carlos
Lactobacillus
pollos de engorde
probióticos
aislamiento
amoníaco
Lactobacillus
broiler
probiotics
isolation
ammonia
title_short IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
title_full IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
title_fullStr IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
title_full_unstemmed IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
title_sort IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
dc.creator.none.fl_str_mv Shiva, Carlos
Jara, Luis M.
author Shiva, Carlos
author_facet Shiva, Carlos
Jara, Luis M.
author_role author
author2 Jara, Luis M.
author2_role author
dc.subject.none.fl_str_mv Lactobacillus
pollos de engorde
probióticos
aislamiento
amoníaco
Lactobacillus
broiler
probiotics
isolation
ammonia
topic Lactobacillus
pollos de engorde
probióticos
aislamiento
amoníaco
Lactobacillus
broiler
probiotics
isolation
ammonia
description The aim of this study was to isolate and identify bacteria of Lactobacillus genus from poultry litter with 1, 2 or 3 uses and low ammonia records. Resistance for conditions at pH 5 and 8 was evaluated as well as the ability to inhibit activity in urease bacteria (Staphylococcus spp and Pseudomona spp) isolated from poultry litter. Seventy three suspected colonies of Lactobacillus were identified by culture in selective media and morphological patterns. Gram staining and catalase test revealed that only 19 strains were of Lactobacillus genus. Furthermore, through PCR was found that 53% corresponded to L. reuteri, 32% to L. plantarum, 10% to L. vaginalis and 5% to L. salivarius. All isolates survived at conditions of pH 5 and 8 and all strains produced some type of active biocin against Staphylococci spp or Pseudomona spp which might be useful as additive to regulate the microbiota in poultry litter.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2579
10.15381/rivep.v24i3.2579
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2579
identifier_str_mv 10.15381/rivep.v24i3.2579
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2579/2256
dc.rights.none.fl_str_mv Derechos de autor 2013 Carlos Shiva, Luis M. Jara
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2013 Carlos Shiva, Luis M. Jara
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 24 Núm. 3 (2013); 307-315
Revista de Investigaciones Veterinarias del Perú; Vol. 24 No. 3 (2013); 307-315
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238224401006592
score 13.959421
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF NATIVE STRAINS OF LACTOBACILLUS ISOLATES FROM POULTRY LITTER
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

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