Effect of hypoosmotic environment on canine semen sperm viability

Descripción del Articulo

The hypoosmotic test is widely used for determining sperm quality in several animal species. The present study aimed to compare the effect of incubation of canine sperm into two hypoosmotic solutions (0 mOsm/l - HOST-s and 150 mOsm/l - HOST) on sperm viability. Fifteen ejaculates (2nd fraction) were...

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Detalles Bibliográficos
Autores: Sánchez R., Alfonso, Zamora D., Patricia
Formato: artículo
Fecha de Publicación:2016
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/11649
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11649
Nivel de acceso:acceso abierto
Materia:canine spermatozoa
hypoosmotic test
sperm viability
espermatozoide canino
prueba hipoosmótica
vitalidad espermática
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spelling Effect of hypoosmotic environment on canine semen sperm viabilityEfecto del Medio Hipoosmótico sobre la Vitalidad Espermática en Semen CaninoSánchez R., AlfonsoZamora D., Patriciacanine spermatozoahypoosmotic testsperm viabilityespermatozoide caninoprueba hipoosmóticavitalidad espermáticaThe hypoosmotic test is widely used for determining sperm quality in several animal species. The present study aimed to compare the effect of incubation of canine sperm into two hypoosmotic solutions (0 mOsm/l - HOST-s and 150 mOsm/l - HOST) on sperm viability. Fifteen ejaculates (2nd fraction) were obtained by digital manipulation from 10dogs and the spermiograms were considered as normals. A 5 μl aliquots per ejaculate were diluted in 45 μl of each of the two hypoosmotic solutions and incubated at 37 °C for 5 and 45 min respectively. Sperm viability was assessed using eosin-nigrosin staining. Results of the hypoosmotic test were 92.1 and 90.1% of dilated spermatozoa for HOST-s and HOST respectively (p<0.05) and the sperm viability was 66.5 and 78.3% of live spermatozoa for HOST-s and HOST respectively (p<0.01). It was concluded that thelower osmolality generates lower sperm viability post-incubation.La prueba hipoosmótica ha sido utilizada ampliamente en la valoración de la calidad seminal en varias especies animales. El presente estudio tuvo la finalidad de comparar el efecto de la incubación de espermatozoides caninos en dos soluciones hipoosmóticas (0 mOsm/l - HOST-s y 150 mOsm/l - HOST) sobre la vitalidad espermática (VE). Se obtuvieron 15 eyaculados (2ª fracción) mediante manipulación digital en 10 perros, cuyos espermiogramas fueron considerados normales. De cada eyaculado se tomaron alícuotas de 5 µl para diluirlas en 45 µl de cada solución hipoosmótica, e incubarlas a 37 ºC por 5 y 45 min, respectivamente. La VE se evaluó mediante tinción eosina-nigrosina. En la prueba hipoosmótica se obtuvo 92.1 y 90.1% de espermatozoides dilatados para HOST-s y HOST, respectivamente (p<0.05) y en la prueba de VE se obtuvo 66.5 y 78.3% de espermatozoides vivos para HOST-s y HOST, respectivamente (p<0.01). Se concluye que la menor osmolaridad genera menor VE posincubación.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2016-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1164910.15381/rivep.v27i2.11649Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 288-293Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 288-2931682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11649/10652Derechos de autor 2016 Alfonso Sánchez R., Patricia Zamora D.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/116492017-02-20T16:35:40Z
dc.title.none.fl_str_mv Effect of hypoosmotic environment on canine semen sperm viability
Efecto del Medio Hipoosmótico sobre la Vitalidad Espermática en Semen Canino
title Effect of hypoosmotic environment on canine semen sperm viability
spellingShingle Effect of hypoosmotic environment on canine semen sperm viability
Sánchez R., Alfonso
canine spermatozoa
hypoosmotic test
sperm viability
espermatozoide canino
prueba hipoosmótica
vitalidad espermática
title_short Effect of hypoosmotic environment on canine semen sperm viability
title_full Effect of hypoosmotic environment on canine semen sperm viability
title_fullStr Effect of hypoosmotic environment on canine semen sperm viability
title_full_unstemmed Effect of hypoosmotic environment on canine semen sperm viability
title_sort Effect of hypoosmotic environment on canine semen sperm viability
dc.creator.none.fl_str_mv Sánchez R., Alfonso
Zamora D., Patricia
author Sánchez R., Alfonso
author_facet Sánchez R., Alfonso
Zamora D., Patricia
author_role author
author2 Zamora D., Patricia
author2_role author
dc.subject.none.fl_str_mv canine spermatozoa
hypoosmotic test
sperm viability
espermatozoide canino
prueba hipoosmótica
vitalidad espermática
topic canine spermatozoa
hypoosmotic test
sperm viability
espermatozoide canino
prueba hipoosmótica
vitalidad espermática
description The hypoosmotic test is widely used for determining sperm quality in several animal species. The present study aimed to compare the effect of incubation of canine sperm into two hypoosmotic solutions (0 mOsm/l - HOST-s and 150 mOsm/l - HOST) on sperm viability. Fifteen ejaculates (2nd fraction) were obtained by digital manipulation from 10dogs and the spermiograms were considered as normals. A 5 μl aliquots per ejaculate were diluted in 45 μl of each of the two hypoosmotic solutions and incubated at 37 °C for 5 and 45 min respectively. Sperm viability was assessed using eosin-nigrosin staining. Results of the hypoosmotic test were 92.1 and 90.1% of dilated spermatozoa for HOST-s and HOST respectively (p<0.05) and the sperm viability was 66.5 and 78.3% of live spermatozoa for HOST-s and HOST respectively (p<0.01). It was concluded that thelower osmolality generates lower sperm viability post-incubation.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11649
10.15381/rivep.v27i2.11649
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11649
identifier_str_mv 10.15381/rivep.v27i2.11649
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11649/10652
dc.rights.none.fl_str_mv Derechos de autor 2016 Alfonso Sánchez R., Patricia Zamora D.
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2016 Alfonso Sánchez R., Patricia Zamora D.
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 288-293
Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 288-293
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238226791759872
score 13.947759
Effect of hypoosmotic environment on canine semen sperm viability
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