Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru

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The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of...

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Detalles Bibliográficos
Autores: Méndez, Carmen R., Vergaray, Germán, Morante, Hilda Y., Flores, Paulo R., Gamboa, Roger A.
Formato: artículo
Fecha de Publicación:2013
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/2680
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2680
Nivel de acceso:acceso abierto
Materia:Escherichia coliO157
H7
bovino
carne molida
factores de virulencia
cattle
ground beef
virulence factors.
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spelling Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-PeruAislamiento y caracterización de Escherichia coliO157:H7 a partir de carne molida de bovino en Lima-PerúMéndez, Carmen R.Vergaray, GermánMorante, Hilda Y.Flores, Paulo R.Gamboa, Roger A.Escherichia coliO157H7bovinocarne molidafactores de virulenciaEscherichia coliO157H7cattleground beefvirulence factors.The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4 (2.05%) strains of E. coliO157 non H7, no virulence factors presented. The study found that the risk potential E. coliO157: H7 affecting the population of LimaEl objetivo del estudio fue aislar y caracterizar E. coliO157:H7 a partir de carne molida fresca de bovino obtenida en diferentes mercados de abastos. Se analizaron 195 muestras; para el aislamiento y enumeración de E. coli se utilizó la técnica del Numero Más probable mediante tubos múltiples; para el aislamiento y caracterización de E. coliO157:H7 el enriquecimiento selectivo y el análisis bioquímico, las colonias características se confirmaron mediante pruebas serológicas. Para determinar la presencia de shigatoxina (stx1, stx2) e intimina (eae A) se empleó la técnica de PCR multiplex en tiempo real y para enterohemolisina la prueba de hemólisis. El 87.18% de la muestras fue positivo para E. coliy el 77.95% presentó un recuento igual o superior a 50 NMP/g. Se obtuvieron 3 (1.54%) cepas de E. coliO157:H7, una stx1 +/ stx2 +/ eaeA – y enterohemolisina -, una stx1 +/ stx2 -/ eaeA + y enterohemolisina – y la otra stx1 -/ stx2 -/ eaeA – y enterohemolisina +. También se obtuvieron 4 cepas (2.05%) de E. coli O157 no H7, ninguna presentó factores de virulencia. El estudio reveló el riesgo potencial de que E. coliO157:H7 afecte a la población de Lima.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2013-08-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/268010.15381/rpb.v20i2.2680Revista Peruana de Biología; Vol. 20 Núm. 2 (2013); 159 - 164Revista Peruana de Biología; Vol. 20 No. 2 (2013); 159 - 1641727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2680/2340Derechos de autor 2013 Carmen R. Méndez, Germán Vergaray, Hilda Y. Morante, Paulo R. Flores, Roger A. Gamboahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/26802020-05-20T19:49:41Z
dc.title.none.fl_str_mv Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
Aislamiento y caracterización de Escherichia coliO157:H7 a partir de carne molida de bovino en Lima-Perú
title Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
spellingShingle Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
Méndez, Carmen R.
Escherichia coliO157
H7
bovino
carne molida
factores de virulencia
Escherichia coliO157
H7
cattle
ground beef
virulence factors.
title_short Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
title_full Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
title_fullStr Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
title_full_unstemmed Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
title_sort Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
dc.creator.none.fl_str_mv Méndez, Carmen R.
Vergaray, Germán
Morante, Hilda Y.
Flores, Paulo R.
Gamboa, Roger A.
author Méndez, Carmen R.
author_facet Méndez, Carmen R.
Vergaray, Germán
Morante, Hilda Y.
Flores, Paulo R.
Gamboa, Roger A.
author_role author
author2 Vergaray, Germán
Morante, Hilda Y.
Flores, Paulo R.
Gamboa, Roger A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Escherichia coliO157
H7
bovino
carne molida
factores de virulencia
Escherichia coliO157
H7
cattle
ground beef
virulence factors.
topic Escherichia coliO157
H7
bovino
carne molida
factores de virulencia
Escherichia coliO157
H7
cattle
ground beef
virulence factors.
description The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4 (2.05%) strains of E. coliO157 non H7, no virulence factors presented. The study found that the risk potential E. coliO157: H7 affecting the population of Lima
publishDate 2013
dc.date.none.fl_str_mv 2013-08-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2680
10.15381/rpb.v20i2.2680
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2680
identifier_str_mv 10.15381/rpb.v20i2.2680
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2680/2340
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 20 Núm. 2 (2013); 159 - 164
Revista Peruana de Biología; Vol. 20 No. 2 (2013); 159 - 164
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.93557
Isolation and characterization of Escherichia coliO157:H7 from ground beef cattle in Lima-Peru
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