PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI

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A glycoprotein from the venom of Bothrops barnetti snake , was purified using gel filtration and ionic exchange chromatography . This protein showed 48 kDa as molecular weight by PAGE-SDS under nonreducing conditions being its carbohydrate content 48% of the total mass protein. This enzyme had activ...

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Detalles Bibliográficos
Autores: Inga A., R., Lazo M., F., Yarlequé Ch., A., Vivas R., D.
Formato: artículo
Fecha de Publicación:2010
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/4600
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4600
Nivel de acceso:acceso abierto
Materia:Venom
snake
amidolytic activity
glycoprotein
Bothrops barnetti
Veneno
serpiente
actividad amidolítica
glicoproteína
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network_name_str Revistas - Universidad Nacional Mayor de San Marcos
repository_id_str
spelling PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTIPURIFICACIÓN Y CARACTERIZACIÓN PARCIAL DE UNA GLICOPROTEÍNA CON ACTIVIDAD AMIDOLÍTICA OBTENIDA DEL VENENO DE LA SERPIENTE PERUANA BOTHROPS BARNETTIInga A., R.Lazo M., F.Yarlequé Ch., A.Vivas R., D.Venomsnakeamidolytic activityglycoproteinBothrops barnettiVenenoserpienteactividad amidolíticaglicoproteínaBothrops barnettiA glycoprotein from the venom of Bothrops barnetti snake , was purified using gel filtration and ionic exchange chromatography . This protein showed 48 kDa as molecular weight by PAGE-SDS under nonreducing conditions being its carbohydrate content 48% of the total mass protein. This enzyme had activity either Benzoil Arginil-p-Nitroanilide (BApNA) and human citrated plasma. In addition this enzyme was strongly inhibited by PMSF and tripsin soybean inhibitor indicating that it is a seninoproeinaseSe ha purificado una glicoproteína del veneno de la serpiente peruana Bothrops barnetti mediante tres pasos cromatográficos que involucran exclusión molecular e intercambio iónico. Mediante PAGE-SDS se determinó que la enzima posee un peso de 48 kDa bajo condiciones no reductoras. El contenido de carbohidratos representa el 48% de la masa total. La enzima presentó actividad sobre el sustrato Benzoil Arginil p Nitroanilida (BApNA) y plasma humano citratado. La enzima fue fuertemente inhibida por el PMSF y el inhibidor de tripsina de soya indicando que se trata de una serinoproteasa.Universidad Nacional Mayor de San Marcos2010-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4600Revista Peruana de Química e Ingeniería Química; Vol. 13 No. 2 (2010); 22-30Revista Peruana de Química e Ingeniería Química; Vol. 13 Núm. 2 (2010); 22-301609-75991726-2208reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4600/3677Derechos de autor 2010 R. Inga A., F. Lazo M., A. Yarlequé Ch., D. Vivas R.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/46002020-05-11T13:14:50Z
dc.title.none.fl_str_mv PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
PURIFICACIÓN Y CARACTERIZACIÓN PARCIAL DE UNA GLICOPROTEÍNA CON ACTIVIDAD AMIDOLÍTICA OBTENIDA DEL VENENO DE LA SERPIENTE PERUANA BOTHROPS BARNETTI
title PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
spellingShingle PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
Inga A., R.
Venom
snake
amidolytic activity
glycoprotein
Bothrops barnetti
Veneno
serpiente
actividad amidolítica
glicoproteína
Bothrops barnetti
title_short PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
title_full PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
title_fullStr PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
title_full_unstemmed PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
title_sort PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
dc.creator.none.fl_str_mv Inga A., R.
Lazo M., F.
Yarlequé Ch., A.
Vivas R., D.
author Inga A., R.
author_facet Inga A., R.
Lazo M., F.
Yarlequé Ch., A.
Vivas R., D.
author_role author
author2 Lazo M., F.
Yarlequé Ch., A.
Vivas R., D.
author2_role author
author
author
dc.subject.none.fl_str_mv Venom
snake
amidolytic activity
glycoprotein
Bothrops barnetti
Veneno
serpiente
actividad amidolítica
glicoproteína
Bothrops barnetti
topic Venom
snake
amidolytic activity
glycoprotein
Bothrops barnetti
Veneno
serpiente
actividad amidolítica
glicoproteína
Bothrops barnetti
description A glycoprotein from the venom of Bothrops barnetti snake , was purified using gel filtration and ionic exchange chromatography . This protein showed 48 kDa as molecular weight by PAGE-SDS under nonreducing conditions being its carbohydrate content 48% of the total mass protein. This enzyme had activity either Benzoil Arginil-p-Nitroanilide (BApNA) and human citrated plasma. In addition this enzyme was strongly inhibited by PMSF and tripsin soybean inhibitor indicating that it is a seninoproeinase
publishDate 2010
dc.date.none.fl_str_mv 2010-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4600
url https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4600
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/quim/article/view/4600/3677
dc.rights.none.fl_str_mv Derechos de autor 2010 R. Inga A., F. Lazo M., A. Yarlequé Ch., D. Vivas R.
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2010 R. Inga A., F. Lazo M., A. Yarlequé Ch., D. Vivas R.
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos
dc.source.none.fl_str_mv Revista Peruana de Química e Ingeniería Química; Vol. 13 No. 2 (2010); 22-30
Revista Peruana de Química e Ingeniería Química; Vol. 13 Núm. 2 (2010); 22-30
1609-7599
1726-2208
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.936249
PURIFICATION AND PARTIAL CHARACTERIZATION OF A GLYCOPROTEIN WITH AMIDOLYTIC ACTIVITY OBTAINED FROM SNAKE VENOM BOTHROPS BARNETTI
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