Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931)
Descripción del Articulo
The aim of the present study was to evaluate the antagonistic activity of a collection of 24 actinomycetes of marine sediment against wild species of Vibrio. Actinomycetes were characterized by their growth in Marine Agar and their extracellular enzymatic activity on different substrates. The antago...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2018 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/14499 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14499 |
| Nivel de acceso: | acceso abierto |
| Materia: | probióticos antagonistas acuicultura Vibrio langostino blanco probiotics antagonists aquaculture white shrimp |
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Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) Caracterización de actinomicetos de sedimento marino y su actividad antagonista frente a Vibrio sp aislados de «langostino blanco» Litopenaeus vannamei (Boone, 1931) |
| title |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) |
| spellingShingle |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) Tarazona J., Ulrike probióticos antagonistas acuicultura Vibrio langostino blanco probiotics antagonists aquaculture Vibrio white shrimp |
| title_short |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) |
| title_full |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) |
| title_fullStr |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) |
| title_full_unstemmed |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) |
| title_sort |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931) |
| dc.creator.none.fl_str_mv |
Tarazona J., Ulrike León Q., Jorge Galindo C., Nadia Vallejo, Marisol Marguet, Emilio |
| author |
Tarazona J., Ulrike |
| author_facet |
Tarazona J., Ulrike León Q., Jorge Galindo C., Nadia Vallejo, Marisol Marguet, Emilio |
| author_role |
author |
| author2 |
León Q., Jorge Galindo C., Nadia Vallejo, Marisol Marguet, Emilio |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
probióticos antagonistas acuicultura Vibrio langostino blanco probiotics antagonists aquaculture Vibrio white shrimp |
| topic |
probióticos antagonistas acuicultura Vibrio langostino blanco probiotics antagonists aquaculture Vibrio white shrimp |
| description |
The aim of the present study was to evaluate the antagonistic activity of a collection of 24 actinomycetes of marine sediment against wild species of Vibrio. Actinomycetes were characterized by their growth in Marine Agar and their extracellular enzymatic activity on different substrates. The antagonist activity was determined by in vitro tests against six strains of Vibrio isolated from Litopenaeus vannamei. Actinomycetes with greater antagonism were evaluated by their growth in standard ISP media and observations of microphotographs by scanning electron microscopy (SEM) techniques were done. The Vibrio strains were evaluated and identified by conventional biochemical techniques and confirmed by PCR. The results indicated that all actinomycetes showed at least some extracellular multi-enzymatic activity, predominating those that hydrolyze starch (79%). Antagonism tests indicated that 54% (13/24) of actinomycetes had anti-Vibrio activity, being the strains M10-77 and M11-116 (B) those that produced the largest inhibition halos. On the other hand, the sequence-based identification of the 16S rRNA gene corroborated the relationship of the isolated strains of L. vannamei with the genus Vibrio; likewise, some of these actinobacteria were identified as Streptomyces. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-05-31 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14499 10.15381/rivep.v29i2.14499 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14499 |
| identifier_str_mv |
10.15381/rivep.v29i2.14499 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14499/12849 https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14499/13969 |
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https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/4.0 |
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openAccess |
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application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 2 (2018); 676-691 Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 2 (2018); 676-691 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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1795238229471920128 |
| spelling |
Characterization of actinomycetes of marine sediment and their antagonistic activity against Vibrio sp. isolated from «whiteleg shrimp» Litopenaeus vannamei (Boone, 1931)Caracterización de actinomicetos de sedimento marino y su actividad antagonista frente a Vibrio sp aislados de «langostino blanco» Litopenaeus vannamei (Boone, 1931)Tarazona J., UlrikeLeón Q., JorgeGalindo C., NadiaVallejo, MarisolMarguet, EmilioprobióticosantagonistasacuiculturaVibriolangostino blancoprobioticsantagonistsaquacultureVibriowhite shrimpThe aim of the present study was to evaluate the antagonistic activity of a collection of 24 actinomycetes of marine sediment against wild species of Vibrio. Actinomycetes were characterized by their growth in Marine Agar and their extracellular enzymatic activity on different substrates. The antagonist activity was determined by in vitro tests against six strains of Vibrio isolated from Litopenaeus vannamei. Actinomycetes with greater antagonism were evaluated by their growth in standard ISP media and observations of microphotographs by scanning electron microscopy (SEM) techniques were done. The Vibrio strains were evaluated and identified by conventional biochemical techniques and confirmed by PCR. The results indicated that all actinomycetes showed at least some extracellular multi-enzymatic activity, predominating those that hydrolyze starch (79%). Antagonism tests indicated that 54% (13/24) of actinomycetes had anti-Vibrio activity, being the strains M10-77 and M11-116 (B) those that produced the largest inhibition halos. On the other hand, the sequence-based identification of the 16S rRNA gene corroborated the relationship of the isolated strains of L. vannamei with the genus Vibrio; likewise, some of these actinobacteria were identified as Streptomyces.El objetivo principal del presente estudio fue evaluar la actividad antagonista de una colección de 24 actinomicetos de sedimento marino frente a especies silvestres de Vibrio. Los actinomicetos fueron caracterizados por su crecimiento en Agar Marino y su actividad enzimática extracelular sobre diferentes sustratos. La actividad antagonista se evaluó mediante pruebas in vitro frente a seis cepas de Vibrio aisladas de Litopenaeus vannamei. Los actinomicetos de mayor antagonismo fueron evaluados por su crecimiento en medios estándares ISP y se hicieron observaciones de microfotografías por técnicas de microscopía electrónica de barrido (MEB). Las cepas de Vibrio fueron evaluadas e identificadas mediante técnicas bioquímicas convencionales y confirmadas por PCR. Los resultados indicaron que la totalidad de actinomicetos mostraron al menos alguna actividad multi-enzimática extracelular, predominando las que hidrolizan almidón (79%). Las pruebas de antagonismo señalaron que el 54% (13/24) de actinomicetos tuvieron actividad anti-Vibrio, siendo las cepas M10-77 y M11-116 (B) las que produjeron los halos de inhibición de mayor tamaño. Por otro lado, la identificación basada en secuencia del gen 16S ARNr corroboró la relación de las cepas aisladas de L. vannamei con el género Vibrio; asimismo, algunas de estas actinobacterias fueron identificadas como Streptomyces.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2018-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1449910.15381/rivep.v29i2.14499Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 2 (2018); 676-691Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 2 (2018); 676-6911682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14499/12849https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14499/13969Derechos de autor 2018 Ulrike Tarazona J., Jorge León Q., Nadia Galindo C., Marisol Vallejo, Emilio Marguethttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/144992018-12-21T09:58:48Z |
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13.924177 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
