Loop-mediated isothermal DNA amplification for asymptomatic malaria detection in challenging field settings: Technical performance and pilot implementation in the Peruvian Amazon

• Autores: Serra-Casas E., Manrique P., Ding X.C., Carrasco-Escobar G., Alava F., Gave A., Rodriguez H., Contreras-Mancilla J., Rosas-Aguirre A., Speybroeck N., González I.J., Rosanas-Urgell A., Gamboa D.
• Formato: artículo
• Fecha de Publicación: 2017
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/678
• Enlace del recurso: https://hdl.handle.net/20.500.12390/678; https://doi.org/10.1371/journal.pone.0185742
• Nivel de acceso: acceso abierto
• Materia: protozoal DNA; adolescent; adult; Amazon; Article; asymptomatic disease; bright field microscopy; child; controlled clinical trial; controlled study; cross-sectional study; density; diagnostic accuracy; diagnostic kit; diagnostic test accuracy study; diagnostic value; disease carrier; DNA determination; feasibility study; female; field study; gene amplification; human; intermethod comparison; loop mediated isothermal amplification; major clinical study; malaria; malaria falciparum; male; mass screening; mixed infection; molecular diagnosis; parasite density; parasite identification; Peru; https://purl.org/pe-repo/ocde/ford#3.03.12

Loop-mediated isothermal DNA amplification (LAMP) methodology offers an opportunity for point-of-care (POC) molecular detection of asymptomatic malaria infections. However, there is still little evidence on the feasibility of implementing this technique for population screenings in isolated field settings.