Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas]
Descripción del Articulo
The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus se...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2019 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/2739 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/2739 https://doi.org/10.15381/rivep.v30i3.15400 |
| Nivel de acceso: | acceso abierto |
| Materia: | Proteomic Arapaima gigas MALDI TOF/TOF Mass spectrometry PepT1 Peptide transporter http://purl.org/pe-repo/ocde/ford#3.02.27 |
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CONC_4459339710768498e085e609fe0c2b80 |
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oai:repositorio.concytec.gob.pe:20.500.12390/2739 |
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CONC |
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CONCYTEC-Institucional |
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4689 |
| dc.title.none.fl_str_mv |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] |
| title |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] |
| spellingShingle |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] Toledo O.E. Proteomic Arapaima gigas MALDI TOF/TOF Mass spectrometry PepT1 Peptide transporter http://purl.org/pe-repo/ocde/ford#3.02.27 |
| title_short |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] |
| title_full |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] |
| title_fullStr |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] |
| title_full_unstemmed |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] |
| title_sort |
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas] |
| author |
Toledo O.E. |
| author_facet |
Toledo O.E. Castañeda A.E. Feria M.A. Masías P. Cueva M. Motte E. |
| author_role |
author |
| author2 |
Castañeda A.E. Feria M.A. Masías P. Cueva M. Motte E. |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Toledo O.E. Castañeda A.E. Feria M.A. Masías P. Cueva M. Motte E. |
| dc.subject.none.fl_str_mv |
Proteomic |
| topic |
Proteomic Arapaima gigas MALDI TOF/TOF Mass spectrometry PepT1 Peptide transporter http://purl.org/pe-repo/ocde/ford#3.02.27 |
| dc.subject.es_PE.fl_str_mv |
Arapaima gigas MALDI TOF/TOF Mass spectrometry PepT1 Peptide transporter |
| dc.subject.ocde.none.fl_str_mv |
http://purl.org/pe-repo/ocde/ford#3.02.27 |
| description |
The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression. © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved. |
| publishDate |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/2739 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.15381/rivep.v30i3.15400 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-85073190489 |
| url |
https://hdl.handle.net/20.500.12390/2739 https://doi.org/10.15381/rivep.v30i3.15400 |
| identifier_str_mv |
2-s2.0-85073190489 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Peru |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.uri.none.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
| instname_str |
Consejo Nacional de Ciencia Tecnología e Innovación |
| instacron_str |
CONCYTEC |
| institution |
CONCYTEC |
| reponame_str |
CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
| repository.name.fl_str_mv |
Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1844883023662678016 |
| spelling |
Publicationrp07314600rp07316600rp07313600rp06333600rp07315600rp07317600Toledo O.E.Castañeda A.E.Feria M.A.Masías P.Cueva M.Motte E.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2019https://hdl.handle.net/20.500.12390/2739https://doi.org/10.15381/rivep.v30i3.154002-s2.0-85073190489The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression. © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengUniversidad Nacional Mayor de San MarcosRevista de Investigaciones Veterinarias del Peruinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/ProteomicArapaima gigas-1MALDI TOF/TOF-1Mass spectrometry-1PepT1-1Peptide transporter-1http://purl.org/pe-repo/ocde/ford#3.02.27-1Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas]info:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#20.500.12390/2739oai:repositorio.concytec.gob.pe:20.500.12390/27392024-05-30 16:19:48.721https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="bd6da2ba-63d4-4bb9-a506-1c2ade851d34"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas]</Title> <PublishedIn> <Publication> <Title>Revista de Investigaciones Veterinarias del Peru</Title> </Publication> </PublishedIn> <PublicationDate>2019</PublicationDate> <DOI>https://doi.org/10.15381/rivep.v30i3.15400</DOI> <SCP-Number>2-s2.0-85073190489</SCP-Number> <Authors> <Author> <DisplayName>Toledo O.E.</DisplayName> <Person id="rp07314" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Castañeda A.E.</DisplayName> <Person id="rp07316" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Feria M.A.</DisplayName> <Person id="rp07313" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Masías P.</DisplayName> <Person id="rp06333" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Cueva M.</DisplayName> <Person id="rp07315" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Motte E.</DisplayName> <Person id="rp07317" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Universidad Nacional Mayor de San Marcos</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by/4.0/</License> <Keyword>Proteomic</Keyword> <Keyword>Arapaima gigas</Keyword> <Keyword>MALDI TOF/TOF</Keyword> <Keyword>Mass spectrometry</Keyword> <Keyword>PepT1</Keyword> <Keyword>Peptide transporter</Keyword> <Abstract>The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression. © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
| score |
13.377112 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
