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1
artículo
Publicado 2021
Enlace

El presente trabajo de investigación tiene como objetivo diseñar y construir un biorreactor batch tipo tanque agitado y aireado con capacidad de un litro para la producción de proteína unicelular. Para su diseño y construcción se empleó un software que genera las especificaciones ideales para este tipo de trabajo, materiales y accesorios fácilmente manipulables y esterilizables a base de vidrio, tapones de caucho para uso químico y mangueras de plástico, el sistema de agitación se construyó con materiales de acero inoxidable más la adición de un motor de paso de 12 V. El biorreactor construido garantiza la esterilidad del sistema, una buena transferencia de oxígeno, calor y masa, al mismo tiempo que genera un mezclado uniforme tanto de la suspensión celular como del caldo fermentativo. Este tipo de biorreactor se diseñó con el propósito de generar las condiciones ideal...
2
artículo
Publicado 2018
Enlace

Puya trianae is characterized for developing a colorful inflorescence, which turned it into an ornamental plant. Is not currently considered threatened, due to the fast habitat deterioration, populations survival is threatened; due to the last, we developed a micropropagation protocol, focused on its conservation and mass production for ornamental purpose, as well as the chromosome counting. For multiplication, seedlings from germinated seeds were used in vitro and we made four tests to determine the type, concentration and time of exposure of some regulators, as well as the partial or total explants excised. Throughout the rooting phase, shoots were grown in MS/2 alone and/or supplemented with IBA and NAA; the acclimatization of the obtained seedlings was carried out in different mixtures land:peat:capote, in a growth room and a greenhouse. Buds and shoots that finished their develop...
3
artículo
Publicado 2018
Enlace

Puya trianae se caracteriza por desarrollar una vistosa inflorescencia que la caracteriza como planta de uso ornamental. Aunque actualmente no se considera amenazada, debido al rápido deterioro de los ecosistemas donde habita, la supervivencia de sus poblaciones sí está amenazada; por dichas razones se desarrolló un protocolo de micropropagación, aplicable para su conservación y para producción masiva de plántulas con fines ornamentales, así como a la determinación de su número cromosómico. Para la multiplicación se utilizaron plántulas provenientes de semillas germinadas in vitro realizando cuatro ensayos para determinar tipo, concentración y tiempo de exposición de algunos reguladores, además de la escisión parcial o total de explantes. Durante la fase de enraizamiento se cultivaron brotes en MS/2 solo y/o suplementado con AIB y ANA; la aclimatización de las plántul...
4
tesis de grado
Publicado 2003
Enlace

factor involved in controlled muscle of shrimp drying permits process optimisation and assists in design of suitable dryers. The study undertook two stages: first a thermal pre-treatment submerging the whole shrimp in water at 90ºC for 90 seconds, were the orange colour and textura was improved. Was used then the sample underwent osmotic dehydration in ternary solution of 20% suerosa and 20%of NaCI ( % weight) under agitation at 120 rpm for 30 minutes to temperatura of 20ºC. The second stage was the drying where the experimental curves were obtained in laboratory dryer under different drying conditions from 40, 50 and 60ºC , and air velocity from 0.5, 0.8 and 1.1 m/s. during this whole process the time of drying was controlled at regular time in someway, the loss weight produced by the evaporation of water contained in the muscle was controlled. With this parameter the product was obt...
5
artículo
Publicado 2016
Enlace

The authors are grateful to the São Paulo Research Foundation (FAPESP, Brazil) for funding the project no. 2014/16998-3 and JC Pereira B.Sc. scholarship (2014/26433-3); the National Council for Scientific and Technological Development (CNPq, Brazil) for funding the project no. 401004/2014-7 and MD Matta Jr. post-doctoral fellowship (158545/2015-0); Cienciactiva for the A.C. Miano Ph.D. scholarship, from the “Consejo Nacional de Ciencia, Tecnología e Inovación Tecnológica” (CONCYTEC, Peru; Contract 272-2015-FONDECYT) and Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil) for the N Castanha M.Sc. scholarship. The authors are also grateful to the “Núcleo de Apoio à Pesquisa em Microscopia Eletrônica Aplicada à Pesquisa Agropecuária” (NAP/MEPA-ESALQ/USP) for the support and facilities of Electronic Microscopy. By describing an important process fo...
6
tesis de grado
Publicado 2022
Enlace

Drying is a method to preserve fruits, vegetables and fish by reducing their volume, their weight. Drying involves the application of energy to vaporize and mobilize the moisture content within porous products. During this process, heat and mass transfer occur simultaneously. The quality of dehydrated fruits, vegetables, and aromatic herbs is a problem closely related to the development and optimization of new drying techniques. This research work addresses the common drying methods applied to fruits, grains, fish and nutrients in relation to possible options to improve the quality of dried products using different drying techniques or their combination. In general, the drying technique is different for each of the materials studied and specific conditions should be recommended after a proper evaluation of the drying protocols. However, a novel or combined technique must ensure a high qu...