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integration processes » integration process (Expander búsqueda), interaction processes (Expander búsqueda), innovation processes (Expander búsqueda)
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1
artÃculo
Construction of a vector for stable chromosomal integration of a Bacillus licheniformis phytase gene
Publicado 2009
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Phytases are a special class of phosphatases that catalyze the sequential hydrolysis of phytate. The inability of plants to utilize phosphorous from soil phytates is due to the low phytase activity in plant roots. Soil microorgan- isms play an important role in the processes that affect the transformation of phosphate containing compounds. Many of them can solubilize phosphorus from phytates, by means of the liberation of phytases. This process allows the mobilization of phosphorus towards the plants and a better utilization of this nutrient. Nevertheless, many soil bacteria lack gene coding for these enzymes, which diminishes the availability of this element in soil. One alternative to obtain improved rhizobacteria in relation to their capacity to solubilize soil phytates is by their genetic transformation with genes that codify for those enzymes. In this work, the gene phyL from B. lic...
2
artÃculo
Publicado 2023
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Talides basistrigata Eaton, 1932, originally described from Peru, was subsequently transferred to the genus Hylephila Billberg, 1820 by Evans (1955) and considered a subspecies of H. phyleus (Drury, 1773), arguing that basistrigata inhabits drier environments than phyleus. Currently, the taxonomic status proposed by Evans is retained. The aim of this study is to reinstate the taxonomic status of valid species for basistrigata based on an integrative analysis considering morphological, ecological, and genetic aspects. Genitalic structures were examined in specimens of both basistrigata and phyleus, mitochondrial COI gene sequences were obtained for both species, and field data were collected to understand their oviposition behaviour in the vicinity of Arequipa city, southwestern Peru. As a result of the morphological examination, notable differences were found between the male genitalia s...
3
artÃculo
Publicado 2009
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Las fitasas son una clase especial de fosfatasas que catalizan la hidrólisis secuencial del fitato. La incapacidad de las plantas para utilizar el fósforo a partir de los fitatos del suelo es debido a la baja actividad de fitasas en sus raÃces. Los microorganismos del suelo juegan un importante papel en los procesos que afectan la trans- formación de los compuestos fosforados. Muchos de ellos pueden solubilizar el fósforo a partir de los fitatos, mediante la liberación de fitasas. Este proceso permite la movilización del fósforo hacia las plantas y un mejor aprovechamiento de este nutriente. Sin embargo, muchas bacterias carecen de los genes que codifican para estas enzimas, lo que disminuye la disponibilidad de este elemento en el suelo. Una alternativa es mejorar las rizobacterias en cuanto a su capacidad de solubilizar los fitatos del suelo, mediante la transformación genéti...
4
tesis de maestrÃa
Publicado 2024
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La tuberculosis, cuyo agente causal es Mycobacterium tuberculosis (MTB), presenta dos tipos de infección: una activa con una alta tasa de mortalidad, y otra inactiva denominada infección latente de tuberculosis (ILTB), con una elevada prevalencia que alcanza a la cuarta parte de la población mundial. Durante la ILTB, MTB se encuentra en estado de dormancia, caracterizado por una baja actividad metabólica. Ante un debilitamiento del sistema inmunológico del huésped, el patógeno puede reactivarse y provocar una infección activa. Sin embargo, hasta la fecha se desconocen los mecanismos moleculares involucrados en la transición de la dormancia a la reactivación. Las redes booleanas permiten modelar sistemas complejos y sus atractores representan estados estables del sistema que pueden asociarse a estados celulares, por lo tanto, pueden ser validados con datos experimentales de expr...
5
artÃculo
Publicado 2014
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In Lima, informal settlements emerge from the construction of straw houses in the desert under poverty and precarious conditions. However, after seven decades of existence, they have transformed into relatively integrated neighborhoods in the city, with considerable level of development. This article describes how an urban fabric emerges within inverted planning processes, from large to small scales, from the city to the house (urbanization-division-consruction), being this inversion the engine of its development. Straw houses, the only material that enables communities to settle in the territory, apart from offering shelter, have become a strategy for city making. Housing is either a workshop or a store, a contribution to the urban fabric; it also changes its functions according to the needs of dwellers; housing expands as the neighborhood densifi es, transforming its typology (from hou...
6
tesis doctoral
Publicado 2015
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Actualmente se han desarrollado trabajos sobre la criopreservación de espermatozoides epididimarios de alpaca, demostrando disminución en los parámetros de viabilidad, motilidad e integridad de la membrana plasmática luego del proceso de descongelación, debido principalmente a especies de oxÃgeno reactivas producidas durante el proceso de congelamiento. El objetivo de este estudio fue evaluar el efecto de la criopreservación sobre las proteÃnas antioxidantes GPX1 y GPX4 de espermatozoides del epidÃdimo de alpaca, usamos muestras (n: 30) de espermatozoides provenientes de la cola del epidÃdimo, se usó un método de congelamiento lento usando dimetilacetamida (0,375 M) como agente crioprotector, analizamos los parámetros de la motilidad, viabilidad e integridad de la membrana plasmática antes y después del proceso de congelamiento; asà mismo evaluamos los extractos de proteÃ...
7
otro
Publicado 2018
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The work of professional proficiency had the title "I feel part of my family", whose development of the learning session has been based on bibliographical references and other sources of consultation, as well as with the application of knowledge acquired in the academic years of my life vocational training. My conclusions summarize that: the end of the family is not only to meet the social, economic and spiritual needs of the adolescent, but also that he feels an important part of his family, regardless of the type of constitution he may have, supporting the time your self-esteem._x000D_ The competence developed in the present class corresponds to "Build your Identity", in which students must be recognized as a member of a specific sociocultural community and have a sense of permanence to their family._x000D_ The didactic processes allow organizing better the learning of the students, or...
8
tesis de grado
Publicado 2020
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El presente trabajo de suficiencia profesional expone demostraciones del cumplimiento del perfil de egreso de la carrera de biologÃa de la UPCH por parte de Daniel Guerra Giraldez. Los aportes cientÃficos cubren la estructura y paisaje energético del inicio de la transcripción, los efectos del ppGpp en la iniciación y la elongación, y la caracterización de la dinámica de la enzima ARN polimerasa de M. tuberculosis (MtbRNAP). Combinando el uso de pinzas ópticas, nucleótidos marcados, microscopÃa de fuerza atómica (AFM), y fluorescencia, se ha demostrado el rol preponderante del enrollamiento como determinante de la estabilidad del complejo de iniciación de la transcripción (RPo) en E. coli, donde resulta antagonista a la acción de ppGpp y catalizador de la tanto la apertura del ADN como del escape de promotor. Se discute las perspectivas de estudio sobre la interacción ent...
9
otro
Publicado 2019
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The work of professional sufficiency was entitled Family Functions, whose development of the_x000D_ learning session has been based on bibliographic references and other sources of consultation, as_x000D_ well as with the application of knowledge acquired in the academic years of my professional training._x000D_ My conclusions summarize that: the family is the most important thing that each human being has,_x000D_ the best that GOD has given us, and above all the kindness and understanding that we find in her, of_x000D_ course when we know how to take care of her and respect first and foremost because Sometimes we_x000D_ think that the family we have is not adequate, that they do not know how to understand us but you_x000D_ are wrong because the family we have is the best of all and that the basis that a family constitutes a_x000D_ couple and the children they have procreated , we will r...
10
tesis de maestrÃa
Publicado 2024
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Amazon forests play a pivotal role in maintaining global ecological balance and are recognized as essential in mitigating climate change impacts. Notably, their soils constitute the largest carbon reservoirs globally and provide a spectrum of crucial ecosystem services. Soil microorganisms serve as primary metabolic drivers, enabling the persistence of carbon within the soil matrix as organic matter. This process is critically modulated by the balance of macronutrients, particularly nitrogen and phosphorus. The efficiency of these metabolic processes, especially those linked to biogeochemical cycling, is largely contingent upon the availability of micronutrients, including essential vitamins. Moreover, external factors such as land-use change and pollution (e.g., artisanal gold mining) may alter microbial community functionality by applying selective pressures on specific microbial popul...
11
artÃculo
Publicado 2025
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Objetive: This integrative review with a descriptive approach aimed to describe the available scientific information on the methods of antifungal identification and susceptibility used to characterize Candida isolates from blood cultures in patients diagnosed with COVID-19. Methods: A systematic search of primary publications was conducted in databases including MEDLINE/PubMed and the Cochrane Library. Specific inclusion and exclusion criteria were established to select studies conducted between 2020 and 2023. Case reports and series, as well as retrospective and prospective analytical studies, were included to describe molecular methods for the identification of Candida species, antifungal susceptibility testing, and the assessment of clonality and resistance genes. Our review process was based on the Whittemore and Knafl model. Results: A total of 446 studies were identified, of which ...
