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artículo
Objective: Mutations in the ABCB1 gene have an impact on the response to various drugs and may be associated with the development of neoplasms. This study explores and compares the frequency distribution of the 1236T>C polymorphism in theABCB1 gene among nine Peruvian populations from different geographical origins and altitudes, as well as the genotype/allele frequencies reported in populations from various continents worldwide. Materials and methods: Sampling was carried out in the following departments and localities: Lima (n = 8), Loreto–Andoas (n = 16), San Martín–Lamas (n = 16), Puno–Uros (n = 7), Puno–Taquile (n = 5), Arequipa–Chivay (n = 10), Arequipa–Cabanaconde (n = 6), Ancash–Parobamba (n = 10), and Apurímac–Andahuaylas (n = 10). Biological samples (peripheral blood and/or epithelial cells) were collected via buccal swabs. Deoxyribonucleic acid (DNA) was e...
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artículo
The Vaccinium genus consists of a variety of berries with high nutritious components consumed worldwide leading to the development of micropropagation protocols to supply the demand. Mortiño, the Andean Blueberry (Vaccinium floribundum Kunth) is a wild berry that grows in high-altitude grasslands with nutritious and commercial potential in Ecuador. In this study, the use of PPMTM (Plant Preservative Mixture™) was effective controlling contamination for the in vitro establishment of Vaccinium floribundum Kunth in contrast to a conventional method using EtOH and Clorox. Stems were defoliated and cut into 1 cm segments, then immersed in liquid MS (Murashige & Skoog) supplemented with 5% v/v PPMTM without pH adjustment for 5 hours under constant shaking. After immersion, segments were transferred to flasks containing WPM (Woody Plant Media) medium supplemented with an additional 2 mlL...
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tesis de grado
Universidad Nacional Agraria La Molina. Facultad de Ciencias. Departamento Académico de Biología