Identification of Proteins Associated with the Formation of Oral Biofilms
Descripción del Articulo
Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confi...
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2021 |
| Institución: | Universidad Privada San Juan Bautista |
| Repositorio: | UPSJB-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.upsjb.edu.pe:upsjb/3020 |
| Enlace del recurso: | http://repositorio.upsjb.edu.pe/handle/upsjb/3020 |
| Nivel de acceso: | acceso abierto |
| Materia: | Dental Plaque Fusobacterium nucleatum Streptococcus gordonii Genomics. |
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| dc.title.es_PE.fl_str_mv |
Identification of Proteins Associated with the Formation of Oral Biofilms |
| title |
Identification of Proteins Associated with the Formation of Oral Biofilms |
| spellingShingle |
Identification of Proteins Associated with the Formation of Oral Biofilms Alvino Vales 5, María Isabel Dental Plaque Fusobacterium nucleatum Streptococcus gordonii Genomics. |
| title_short |
Identification of Proteins Associated with the Formation of Oral Biofilms |
| title_full |
Identification of Proteins Associated with the Formation of Oral Biofilms |
| title_fullStr |
Identification of Proteins Associated with the Formation of Oral Biofilms |
| title_full_unstemmed |
Identification of Proteins Associated with the Formation of Oral Biofilms |
| title_sort |
Identification of Proteins Associated with the Formation of Oral Biofilms |
| author |
Alvino Vales 5, María Isabel |
| author_facet |
Alvino Vales 5, María Isabel Millones-Gómez1, Pablo Alejandro Reyma Evelyn, Bacilio Amaranto2 Maurtua Torres2, Dora Jesús Calla-Poma3, Roger Damaso Requena-Mendizabal4, Margarita F. Calla-Poma6, Rubén |
| author_role |
author |
| author2 |
Millones-Gómez1, Pablo Alejandro Reyma Evelyn, Bacilio Amaranto2 Maurtua Torres2, Dora Jesús Calla-Poma3, Roger Damaso Requena-Mendizabal4, Margarita F. Calla-Poma6, Rubén |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Alvino Vales 5, María Isabel Millones-Gómez1, Pablo Alejandro Reyma Evelyn, Bacilio Amaranto2 Maurtua Torres2, Dora Jesús Calla-Poma3, Roger Damaso Requena-Mendizabal4, Margarita F. Calla-Poma6, Rubén |
| dc.subject.es_PE.fl_str_mv |
Dental Plaque Fusobacterium nucleatum Streptococcus gordonii Genomics. |
| topic |
Dental Plaque Fusobacterium nucleatum Streptococcus gordonii Genomics. |
| description |
Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion. |
| publishDate |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2021-06-11T22:07:33Z |
| dc.date.available.none.fl_str_mv |
2021-06-11T22:07:33Z |
| dc.date.issued.fl_str_mv |
2021 |
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info:eu-repo/semantics/article |
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article |
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http://repositorio.upsjb.edu.pe/handle/upsjb/3020 |
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http://repositorio.upsjb.edu.pe/handle/upsjb/3020 |
| dc.language.iso.es_PE.fl_str_mv |
eng |
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eng |
| dc.rights.es_PE.fl_str_mv |
info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 United States |
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licenses/by-nc-nd/3.0/us/ |
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http://creativecommons.org/licenses/by-nc-nd/3.0/us/ |
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openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 United States licenses/by-nc-nd/3.0/us/ http://creativecommons.org/licenses/by-nc-nd/3.0/us/ |
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application/pdf |
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Association of Support to Oral Health Research - APESB |
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Universidad Privada San Juan Bautista Repositorio Institucional UPSJB |
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UPSJB-Institucional |
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Alvino Vales 5, María IsabelMillones-Gómez1, Pablo AlejandroReyma Evelyn, Bacilio Amaranto2Maurtua Torres2, Dora JesúsCalla-Poma3, Roger DamasoRequena-Mendizabal4, Margarita F.Calla-Poma6, Rubén2021-06-11T22:07:33Z2021-06-11T22:07:33Z2021http://repositorio.upsjb.edu.pe/handle/upsjb/3020Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.Submitted by Manuel Cabrera (manuel.cabrera@upsjb.edu.pe) on 2021-06-11T22:07:33Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) PI - FCS- E - María Isabel Alvino Vales.pdf: 3564139 bytes, checksum: 0bbbf8864e49ee3c690a37daf1a6c91a (MD5)Made available in DSpace on 2021-06-11T22:07:33Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) PI - FCS- E - María Isabel Alvino Vales.pdf: 3564139 bytes, checksum: 0bbbf8864e49ee3c690a37daf1a6c91a (MD5) Previous issue date: 2021PEapplication/pdfengAssociation of Support to Oral Health Research - APESBinfo:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivs 3.0 United Stateslicenses/by-nc-nd/3.0/us/http://creativecommons.org/licenses/by-nc-nd/3.0/us/Universidad Privada San Juan BautistaRepositorio Institucional UPSJBreponame:UPSJB-Institucionalinstname:Universidad Privada San Juan Bautistainstacron:UPSJBDental PlaqueFusobacterium nucleatumStreptococcus gordoniiGenomics.Identification of Proteins Associated with the Formation of Oral Biofilmsinfo:eu-repo/semantics/articleLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.upsjb.edu.pe/bitstream/upsjb/3020/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81232http://repositorio.upsjb.edu.pe/bitstream/upsjb/3020/2/license_rdfbb87e2fb4674c76d0d2e9ed07fbb9c86MD52ORIGINALPI - FCS- E - María Isabel Alvino Vales.pdfPI - FCS- E - María Isabel Alvino Vales.pdfapplication/pdf3564139http://repositorio.upsjb.edu.pe/bitstream/upsjb/3020/1/PI%20-%20FCS-%20E%20-%20Mar%c3%ada%20Isabel%20Alvino%20Vales.pdf0bbbf8864e49ee3c690a37daf1a6c91aMD51upsjb/3020oai:repositorio.upsjb.edu.pe:upsjb/30202022-01-06 20:17:51.45Universidad Privada San Juan Bautistarepositoriovirtual@upsjb.edu.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 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
