Identification of Proteins Associated with the Formation of Oral Biofilms

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Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confi...

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Detalles Bibliográficos
Autores: Alvino Vales5, María Isabel, Millones-Gómez1, Pablo Alejandro, Bacilio Amaranto2, Reyma Evelyn, Maurtuao Torres2, Dora Jesús, Calla-Poma3, Roger Damaso, Requena-Mendizabal4, Margarita F, Calla-Poma6, Rubén
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Privada San Juan Bautista
Repositorio:UPSJB-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.upsjb.edu.pe:upsjb/3073
Enlace del recurso:http://repositorio.upsjb.edu.pe/handle/upsjb/3073
Nivel de acceso:acceso abierto
Materia:Dental Plaque
Fusobacterium nucleatum
Streptococcus gordonii
Genomics
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dc.title.es_PE.fl_str_mv Identification of Proteins Associated with the Formation of Oral Biofilms
title Identification of Proteins Associated with the Formation of Oral Biofilms
spellingShingle Identification of Proteins Associated with the Formation of Oral Biofilms
Alvino Vales5, María Isabel
Dental Plaque
Fusobacterium nucleatum
Streptococcus gordonii
Genomics
title_short Identification of Proteins Associated with the Formation of Oral Biofilms
title_full Identification of Proteins Associated with the Formation of Oral Biofilms
title_fullStr Identification of Proteins Associated with the Formation of Oral Biofilms
title_full_unstemmed Identification of Proteins Associated with the Formation of Oral Biofilms
title_sort Identification of Proteins Associated with the Formation of Oral Biofilms
author Alvino Vales5, María Isabel
author_facet Alvino Vales5, María Isabel
Millones-Gómez1, Pablo Alejandro
Bacilio Amaranto2, Reyma Evelyn
Maurtuao Torres2, Dora Jesús
Calla-Poma3, Roger Damaso
Requena-Mendizabal4, Margarita F
Calla-Poma6, Rubén
author_role author
author2 Millones-Gómez1, Pablo Alejandro
Bacilio Amaranto2, Reyma Evelyn
Maurtuao Torres2, Dora Jesús
Calla-Poma3, Roger Damaso
Requena-Mendizabal4, Margarita F
Calla-Poma6, Rubén
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alvino Vales5, María Isabel
Millones-Gómez1, Pablo Alejandro
Bacilio Amaranto2, Reyma Evelyn
Maurtuao Torres2, Dora Jesús
Calla-Poma3, Roger Damaso
Requena-Mendizabal4, Margarita F
Calla-Poma6, Rubén
dc.subject.es_PE.fl_str_mv Dental Plaque
Fusobacterium nucleatum
Streptococcus gordonii
Genomics
topic Dental Plaque
Fusobacterium nucleatum
Streptococcus gordonii
Genomics
description Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-06-17T04:35:57Z
dc.date.available.none.fl_str_mv 2021-06-17T04:35:57Z
dc.date.issued.fl_str_mv 2021
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv http://repositorio.upsjb.edu.pe/handle/upsjb/3073
url http://repositorio.upsjb.edu.pe/handle/upsjb/3073
dc.language.iso.es_PE.fl_str_mv eng
language eng
dc.rights.es_PE.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.*.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 United States
dc.rights.uri.es_PE.fl_str_mv licenses/by-nc-nd/3.0/us/
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/us/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 United States
licenses/by-nc-nd/3.0/us/
http://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.format.es_PE.fl_str_mv application/pdf
dc.publisher.es_PE.fl_str_mv Association of Support to Oral Health Research - APESB
dc.publisher.country.none.fl_str_mv PE
dc.source.es_PE.fl_str_mv Universidad Privada San Juan Bautista
Repositorio Institucional UPSJB
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instacron:UPSJB
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instacron_str UPSJB
institution UPSJB
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collection UPSJB-Institucional
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spelling Alvino Vales5, María IsabelMillones-Gómez1, Pablo AlejandroBacilio Amaranto2, Reyma EvelynMaurtuao Torres2, Dora JesúsCalla-Poma3, Roger DamasoRequena-Mendizabal4, Margarita FCalla-Poma6, Rubén2021-06-17T04:35:57Z2021-06-17T04:35:57Z2021http://repositorio.upsjb.edu.pe/handle/upsjb/3073Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesionSubmitted by Manuel Cabrera (manuel.cabrera@upsjb.edu.pe) on 2021-06-17T04:35:57Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) PI - FCS - E - María Isabel Alvino Vales.pdf: 3564139 bytes, checksum: 0bbbf8864e49ee3c690a37daf1a6c91a (MD5)Made available in DSpace on 2021-06-17T04:35:57Z (GMT). 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Identification of Proteins Associated with the Formation of Oral Biofilms
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