Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru

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We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%–42%) in 24 culture-confirmed tuberculosis cases and 4.6%...

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Detalles Bibliográficos
Autores: Juan A., Flores, Coit, Julia, Milagros Mendoza, León-Sandoval, Segundo R., Kelika Kond, Leonid Lecca & Molly F, Frank
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Privada San Juan Bautista
Repositorio:UPSJB-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.upsjb.edu.pe:upsjb/2915
Enlace del recurso:http://repositorio.upsjb.edu.pe/handle/upsjb/2915
Nivel de acceso:acceso abierto
Materia:oral swab
real-time polymerase chain reaction
tuberculosis
childhood tuberculosis
diagnostic
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dc.title.es_PE.fl_str_mv Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
title Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
spellingShingle Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
Juan A., Flores
oral swab
real-time polymerase chain reaction
tuberculosis
childhood tuberculosis
diagnostic
title_short Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
title_full Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
title_fullStr Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
title_full_unstemmed Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
title_sort Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
author Juan A., Flores
author_facet Juan A., Flores
Coit, Julia
Milagros Mendoza
León-Sandoval, Segundo R.
Kelika Kond
Leonid Lecca & Molly F, Frank
author_role author
author2 Coit, Julia
Milagros Mendoza
León-Sandoval, Segundo R.
Kelika Kond
Leonid Lecca & Molly F, Frank
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Juan A., Flores
Coit, Julia
Milagros Mendoza
León-Sandoval, Segundo R.
Kelika Kond
Leonid Lecca & Molly F, Frank
dc.subject.es_PE.fl_str_mv oral swab
real-time polymerase chain reaction
tuberculosis
childhood tuberculosis
diagnostic
topic oral swab
real-time polymerase chain reaction
tuberculosis
childhood tuberculosis
diagnostic
description We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%–42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%–13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%–100%).
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2021-02-17T23:55:34Z
dc.date.available.none.fl_str_mv 2021-02-17T23:55:34Z
dc.date.issued.fl_str_mv 2020
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv http://repositorio.upsjb.edu.pe/handle/upsjb/2915
url http://repositorio.upsjb.edu.pe/handle/upsjb/2915
dc.language.iso.es_PE.fl_str_mv eng
language eng
dc.rights.es_PE.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 United States
licenses/by-nc-nd/3.0/us/
http://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.format.es_PE.fl_str_mv application/pdf
dc.publisher.es_PE.fl_str_mv The Pediatric Infectious Disease Journal
dc.publisher.country.none.fl_str_mv PE
dc.source.es_PE.fl_str_mv Universidad Privada San Juan Bautista
Repositorio Institucional UPSJB
dc.source.none.fl_str_mv reponame:UPSJB-Institucional
instname:Universidad Privada San Juan Bautista
instacron:UPSJB
instname_str Universidad Privada San Juan Bautista
instacron_str UPSJB
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spelling Juan A., FloresCoit, JuliaMilagros MendozaLeón-Sandoval, Segundo R.Kelika KondLeonid Lecca & Molly F, Frank2021-02-17T23:55:34Z2021-02-17T23:55:34Z2020http://repositorio.upsjb.edu.pe/handle/upsjb/2915We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%–42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%–13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%–100%).Submitted by Manuel Cabrera (manuel.cabrera@upsjb.edu.pe) on 2021-02-17T23:55:34Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) PI-FCS -TM- Segundo Leon Sandoval -2021.pdf: 714718 bytes, checksum: 2b2d01bf62590c1362593d95a2acb2de (MD5)Made available in DSpace on 2021-02-17T23:55:34Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: bb87e2fb4674c76d0d2e9ed07fbb9c86 (MD5) PI-FCS -TM- Segundo Leon Sandoval -2021.pdf: 714718 bytes, checksum: 2b2d01bf62590c1362593d95a2acb2de (MD5) Previous issue date: 2020application/pdfengThe Pediatric Infectious Disease JournalPEinfo:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivs 3.0 United Stateslicenses/by-nc-nd/3.0/us/http://creativecommons.org/licenses/by-nc-nd/3.0/us/Universidad Privada San Juan BautistaRepositorio Institucional UPSJBreponame:UPSJB-Institucionalinstname:Universidad Privada San Juan Bautistainstacron:UPSJBoral swabreal-time polymerase chain reactiontuberculosischildhood tuberculosisdiagnosticDetection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peruinfo:eu-repo/semantics/articleLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.upsjb.edu.pe/bitstream/upsjb/2915/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81232http://repositorio.upsjb.edu.pe/bitstream/upsjb/2915/2/license_rdfbb87e2fb4674c76d0d2e9ed07fbb9c86MD52ORIGINALPI-FCS -TM- Segundo Leon Sandoval -2021.pdfPI-FCS -TM- Segundo Leon Sandoval -2021.pdfapplication/pdf714718http://repositorio.upsjb.edu.pe/bitstream/upsjb/2915/1/PI-FCS%20-TM-%20Segundo%20Leon%20Sandoval%20-2021.pdf2b2d01bf62590c1362593d95a2acb2deMD51upsjb/2915oai:repositorio.upsjb.edu.pe:upsjb/29152022-01-06 20:15:57.238Universidad Privada San Juan Bautistarepositoriovirtual@upsjb.edu.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Detection of mycobacterium tuberculosis dna in buccal swab samples from children in lima, Peru
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