Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes

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Objective: The principal objective of these investigation was to evaluate, in vitro, bactericidal effect of the ethanolic extract of Piper aduncum (matico) on Streptococcus pyogenes Group A. The ethanolic extract of Piper aduncum was prepared in five differents concentrations: 5%, 25%, 50%, 75% and...

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Detalles Bibliográficos
Autor: Benites Rodríguez, Alondra Nathalie
Formato: tesis de grado
Fecha de Publicación:2017
Institución:Universidad Nacional de Trujillo
Repositorio:UNITRU-Tesis
Lenguaje:español
OAI Identifier:oai:dspace.unitru.edu.pe:20.500.14414/9372
Enlace del recurso:https://hdl.handle.net/20.500.14414/9372
Nivel de acceso:acceso abierto
Materia:Piper aduncum, Extracto etanólico, Streptococcus pyogenes
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dc.title.es_PE.fl_str_mv Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
title Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
spellingShingle Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
Benites Rodríguez, Alondra Nathalie
Piper aduncum, Extracto etanólico, Streptococcus pyogenes
title_short Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
title_full Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
title_fullStr Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
title_full_unstemmed Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
title_sort Efecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenes
author Benites Rodríguez, Alondra Nathalie
author_facet Benites Rodríguez, Alondra Nathalie
author_role author
dc.contributor.advisor.fl_str_mv Mejía Delgado, Elva Manuela
dc.contributor.author.fl_str_mv Benites Rodríguez, Alondra Nathalie
dc.subject.es_PE.fl_str_mv Piper aduncum, Extracto etanólico, Streptococcus pyogenes
topic Piper aduncum, Extracto etanólico, Streptococcus pyogenes
description Objective: The principal objective of these investigation was to evaluate, in vitro, bactericidal effect of the ethanolic extract of Piper aduncum (matico) on Streptococcus pyogenes Group A. The ethanolic extract of Piper aduncum was prepared in five differents concentrations: 5%, 25%, 50%, 75% and 100%; also Penicillin of 10 IU was use as control. Methods: The Kirby Bauer (disc diffusion) method and the minimum inhibitory concentration (IMC) were used to evaluate the antibacterial activity of Piper aduncum on Streptococcus pyogenes Group A. Results: When comparing the inhibition halos according to the Duraffourd scale, Streptococcus pyogenes was highly sensitive to the five concentrations of the ethanolic extract of Piper aduncum like Penicillin but there isn´t significantly different between concentrations. Evaluating MIC, growth of S. pyogenes was inhibit by every kind of concentrations, also all the concentrations show a bactericidal effect. Conclusions: It was determined that the ethanolic extract of Piper aduncum (matico) has bactericidal effect in vitro on the growth of Streptococcus Pyogenes of group A
publishDate 2017
dc.date.accessioned.none.fl_str_mv 2017-12-26T13:28:57Z
dc.date.available.none.fl_str_mv 2017-12-26T13:28:57Z
dc.date.issued.fl_str_mv 2017
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.14414/9372
url https://hdl.handle.net/20.500.14414/9372
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language spa
dc.relation.ispartof.fl_str_mv SUNEDU
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eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/pe/
dc.publisher.es_PE.fl_str_mv Universidad Nacional de Trujillo
dc.source.es_PE.fl_str_mv Universidad Nacional de Trujillo
Repositorio institucional - UNITRU
dc.source.none.fl_str_mv reponame:UNITRU-Tesis
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spelling Mejía Delgado, Elva ManuelaBenites Rodríguez, Alondra Nathalie2017-12-26T13:28:57Z2017-12-26T13:28:57Z2017https://hdl.handle.net/20.500.14414/9372Objective: The principal objective of these investigation was to evaluate, in vitro, bactericidal effect of the ethanolic extract of Piper aduncum (matico) on Streptococcus pyogenes Group A. The ethanolic extract of Piper aduncum was prepared in five differents concentrations: 5%, 25%, 50%, 75% and 100%; also Penicillin of 10 IU was use as control. Methods: The Kirby Bauer (disc diffusion) method and the minimum inhibitory concentration (IMC) were used to evaluate the antibacterial activity of Piper aduncum on Streptococcus pyogenes Group A. Results: When comparing the inhibition halos according to the Duraffourd scale, Streptococcus pyogenes was highly sensitive to the five concentrations of the ethanolic extract of Piper aduncum like Penicillin but there isn´t significantly different between concentrations. Evaluating MIC, growth of S. pyogenes was inhibit by every kind of concentrations, also all the concentrations show a bactericidal effect. Conclusions: It was determined that the ethanolic extract of Piper aduncum (matico) has bactericidal effect in vitro on the growth of Streptococcus Pyogenes of group AObjetivo: El objetivo de la presente investigación fue evaluar el efecto antibacteriano in vitro del extracto etanólico de Piper aduncum (matico) sobre Streptococcus pyogenes Grupo A. Material y Método: Se preparó el extracto etanólico de Piper aduncum en cinco concentraciones: 5%, 25%, 50%, 75% y 100%; además se utilizó como control Penicilina de 10 UI. Se utilizó el método de Kirby Bauer (difusión en disco) y la determinación mínima inhibitoria (CMI) para evaluar la actividad antibacteriana de Piper aduncum sobre Streptococcus pyogenes Grupo A. Resultados: Al comparar los halos de inhibición según la escala de Duraffourd, Streptococcus pyogenes fue sumamente sensible a las cinco concentraciones del extracto etanólico de Piper aduncum obteniendo medidas similares al control penicilina y sin que exista diferencia estadísticamente significativa entre concentraciones. Al evaluar la concentración inhibitoria mínima, se determinó que todas las concentraciones inhibieron el crecimiento de S. pyogenes además que todas tuvieron efecto bactericida. Conclusiones: Se determinó que el extracto etanólico de Piper aduncum (matico) posee efecto bactericida in vitro sobre el crecimiento de Streptococcus Pyogenes del grupo ATesisspaUniversidad Nacional de Trujilloinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/pe/Universidad Nacional de TrujilloRepositorio institucional - UNITRUreponame:UNITRU-Tesisinstname:Universidad Nacional de Trujilloinstacron:UNITRUPiper aduncum, Extracto etanólico, Streptococcus pyogenesEfecto bactericida in vitro de Piper aduncum sobre streptococcus pyogenesinfo:eu-repo/semantics/bachelorThesisSUNEDUTítulo ProfesionalMédicoMedicinaUniversidad Nacional de Trujillo.Facultad de MedicinaLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://dspace.unitru.edu.pe/bitstreams/9401f245-2c02-4c15-9ed0-6f2e7aa24c8e/download8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALBenitesRodriguez_A.pdfBenitesRodriguez_A.pdfapplication/pdf2573668https://dspace.unitru.edu.pe/bitstreams/807ae2b8-fd55-42ef-90ac-2ffa48a3b352/download23e7c0aaf4d3f0ce1d977ffa42a0ad6cMD5120.500.14414/9372oai:dspace.unitru.edu.pe:20.500.14414/93722017-12-26 08:28:57.271http://creativecommons.org/licenses/by-nc-nd/2.5/pe/info:eu-repo/semantics/openAccessopen.accesshttps://dspace.unitru.edu.peRepositorio Institucional - UNITRUrepositorios@unitru.edu.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