“Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”

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The purpose of this experimental study was to determinate the in vitro antibacterial effect of the ethanolic extract of Solanum tuberosum (lyophilized potato: black chuño) against strains of Streptococcus mutans. The susceptibility test was performed, using the well diffusion method tested on the co...

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Detalles Bibliográficos
Autor: Gamboa Mendoza, Lener Albert
Formato: tesis de grado
Fecha de Publicación:2020
Institución:Universidad Nacional de Trujillo
Repositorio:UNITRU-Tesis
Lenguaje:español
OAI Identifier:oai:dspace.unitru.edu.pe:20.500.14414/16616
Enlace del recurso:https://hdl.handle.net/20.500.14414/16616
Nivel de acceso:acceso abierto
Materia:Solanum Tuberosum
Streptococcus mutans
Antibacterianos
https//purl.org/pe-repo/ocde/Ford#3.02.14
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dc.title.es_PE.fl_str_mv “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
title “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
spellingShingle “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
Gamboa Mendoza, Lener Albert
Solanum Tuberosum
Streptococcus mutans
Antibacterianos
https//purl.org/pe-repo/ocde/Ford#3.02.14
title_short “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
title_full “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
title_fullStr “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
title_full_unstemmed “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
title_sort “Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”
author Gamboa Mendoza, Lener Albert
author_facet Gamboa Mendoza, Lener Albert
author_role author
dc.contributor.advisor.fl_str_mv Guardia Méndez, Gustavo
dc.contributor.author.fl_str_mv Gamboa Mendoza, Lener Albert
dc.subject.es_PE.fl_str_mv Solanum Tuberosum
Streptococcus mutans
Antibacterianos
topic Solanum Tuberosum
Streptococcus mutans
Antibacterianos
https//purl.org/pe-repo/ocde/Ford#3.02.14
dc.subject.ocde.none.fl_str_mv https//purl.org/pe-repo/ocde/Ford#3.02.14
description The purpose of this experimental study was to determinate the in vitro antibacterial effect of the ethanolic extract of Solanum tuberosum (lyophilized potato: black chuño) against strains of Streptococcus mutans. The susceptibility test was performed, using the well diffusion method tested on the concentrations of 25%, 50% and 75% of Solanum tuberosum ethanolic extract. All concentrations showed inhibition halos and the sizes increased directly proportional to the concentrations used.To find the Minimum Inhibitory Concentration, the method of dilution in tubes was used, testing the same concentrations and the respective control; of each culture was plated with Mueller Hinton-Blood Agar to determine the Colony Forming Units (CFU's).The results showed that the concentration of 75% of the ethanolic extract of Solanum tuberosum was very sensitive, showing the highest average inhibition halo (16 mm) and the minimum inhibitory concentration was effective in the three concentrations, as it did not present colony-forming units in the different repetitions of the analyzed sample, of these concentrations the numerically lower was 25%. Finally, it is concluded that the ethanolic extract of Solanum tuberosum (black chuño) has antibacterial activity in vitro on the growth of Streptococcus mutans strains
publishDate 2020
dc.date.accessioned.none.fl_str_mv 4/11/2023 13:16
dc.date.available.none.fl_str_mv 4/11/2023 13:16
dc.date.issued.fl_str_mv 2020
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.14414/16616
url https://hdl.handle.net/20.500.14414/16616
dc.language.iso.es_ES.fl_str_mv spa
language spa
dc.relation.ispartof.fl_str_mv SUNEDU
dc.rights.es_PE.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.es_PE.fl_str_mv Universidad Nacional de Trujillo
dc.publisher.country.none.fl_str_mv PE
dc.source.none.fl_str_mv reponame:UNITRU-Tesis
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spelling Guardia Méndez, GustavoGamboa Mendoza, Lener Albert4/11/2023 13:164/11/2023 13:162020https://hdl.handle.net/20.500.14414/16616The purpose of this experimental study was to determinate the in vitro antibacterial effect of the ethanolic extract of Solanum tuberosum (lyophilized potato: black chuño) against strains of Streptococcus mutans. The susceptibility test was performed, using the well diffusion method tested on the concentrations of 25%, 50% and 75% of Solanum tuberosum ethanolic extract. All concentrations showed inhibition halos and the sizes increased directly proportional to the concentrations used.To find the Minimum Inhibitory Concentration, the method of dilution in tubes was used, testing the same concentrations and the respective control; of each culture was plated with Mueller Hinton-Blood Agar to determine the Colony Forming Units (CFU's).The results showed that the concentration of 75% of the ethanolic extract of Solanum tuberosum was very sensitive, showing the highest average inhibition halo (16 mm) and the minimum inhibitory concentration was effective in the three concentrations, as it did not present colony-forming units in the different repetitions of the analyzed sample, of these concentrations the numerically lower was 25%. Finally, it is concluded that the ethanolic extract of Solanum tuberosum (black chuño) has antibacterial activity in vitro on the growth of Streptococcus mutans strainsEl propósito del presente estudio de tipo experimental fue determinar el efecto antibacteriano in vitro del extracto etanólico de Solanum tuberosum (papa liofilizada: chuño negro) frente a cepas de Streptococcus mutans. Se realizó la prueba de susceptibilidad, utilizando el método de difusión en pocitos ensayada sobre las concentraciones de 25%, 50% y 75% de extracto etanólico de Solanum tuberosum. Todas las concentraciones presentaron halos de inhibición y los tamaños aumentaron directamente proporcional a las concentraciones utilizadas.Para hallar la Concentración Mínima Inhibitoria se empleó el método de dilución en tubos, ensayando las mismas concentraciones y el control respectivo; de cada cultivo se sembró en placas con Agar Mueller Hinton – Sangre para determinar las Unidades Formadoras de Colonias (UFC’s).Los resultados mostraron que la concentración del 75% del extracto etanólico de Solanum tuberosum fue muy sensible evidenciando el halo de inhibición promedio más alto (16 mm) y la concentración mínima inhibitoria fue efectiva en las tres concentraciones, al no presentar unidades formadoras de colonias en las distintas repeticiones de la muestra analizada, de estas concentraciones la numéricamente menor fue la del 25%.Finalmente, se concluye que el extracto etanólico de Solanum tuberosum (chuño negro) posee actividad antibacteriana in vitro sobre el crecimiento de cepas de Streptococcus mutansapplication/pdfspaUniversidad Nacional de TrujilloPEinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Solanum TuberosumStreptococcus mutansAntibacterianoshttps//purl.org/pe-repo/ocde/Ford#3.02.14“Efecto antibacteriano del extracto etanólico de Solanum tuberosum (chuño negro) frente a Streptococcus mutans, 2019”info:eu-repo/semantics/bachelorThesisreponame:UNITRU-Tesisinstname:Universidad Nacional de Trujilloinstacron:UNITRUSUNEDUCirujano DentistaEstomatologíaUniversidad Nacional de Trujillo.Facultad de Estomatología17968555https://orcid.org/0000-0002-8637-392946448119911016Palomino Morón, Gladys IsauraBenavides Samander, Gustavo AdolfoHuamaní Muñoz, Balbín Wilderhttp//purl.org/pe-repo/renati/level#títuloProfesionalhttp//purl.org/pe-repo/renati/type#tesisORIGINALTESIS GAMBOA MENDOZA LENER. UNT. 2020 PROTEJIDO.pdfTESIS GAMBOA MENDOZA LENER. UNT. 2020 PROTEJIDO.pdfapplication/pdf5401725https://dspace.unitru.edu.pe/bitstreams/aba9d68c-ee3b-470a-839a-acb1e7b184aa/download35c50d293fece6d64e2aa7ef467c3b2bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://dspace.unitru.edu.pe/bitstreams/dc84e6cf-c7e9-4565-85b6-bf4ce826cdb8/download8a4605be74aa9ea9d79846c1fba20a33MD5220.500.14414/16616oai:dspace.unitru.edu.pe:20.500.14414/166162024-10-21 12:24:02.285http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessopen.accesshttps://dspace.unitru.edu.peRepositorio Institucional - UNITRUrepositorios@unitru.edu.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