Caracterización molecular y patrón de susceptibilidad antimicrobiana en gérmenes productores de b-Lactamasas, en un hospital de tercer nivel en Loreto, Perú

• Autor: Chafloque Vasquez, Ricci Alonso
• Formato: tesis de maestría
• Fecha de Publicación: 2022
• Institución: Universidad Nacional De La Amazonía Peruana
• Repositorio: UNAPIquitos-Institucional
• Lenguaje: español
• OAI Identifier: oai:repositorio.unapiquitos.edu.pe:20.500.12737/8197
• Enlace del recurso: https://hdl.handle.net/20.500.12737/8197
• Nivel de acceso: acceso abierto
• Materia: Enterobacteriaceae; Prevalencia; Escherichia coli; Klebsiella; Beta-Lactamasas; Farmacorresistencia microbiana; https://purl.org/pe-repo/ocde/ford#3.02.27; https://purl.org/pe-repo/ocde/ford#1.06.01

An increase in infections caused by extended-spectrum beta-lactamaseproducing gram-negative bacilli (ESBL) represents a growing problem worldwide. Several studies conducted at the Regional Hospital of Loreto have reported up to 50% prevalence of infections associated with ESBL bacteria. We are currently experiencing a major epidemic of these ESBL-producing gramnegative germs (CTX-M15, SHV, TEM) that emerged from the hospital environment a few years ago and has even migrated to the community environment due to the indiscriminate use of antibiotics. The objective of the study is to identify the phenotypic and molecular character of resistance associated with these infections. Using a descriptive design, the proposed study will describe, using molecular biology techniques, the phenotypic and genotypic characteristics of these beta-lactamase-producing agents. The methodology consists of identifying all the positive cultures of patients hospitalized in the Regional Hospital of Loreto, to whom, using an automated system of "VITEK-2", the bacterial identification and antibiotic susceptibility (phenotype) for E. coli and Klebsiella spp will be carried out. . All cultures belonging to patients who meet the proposed criteria will be included in the study. All cultures with a phenotypic character with ESBL production will have the DNA of the resistance genes extracted and the amplification and naming of the molecular pattern will be carried out in order to determine the genotypic characteristics of the multi-resistant agents in our environment. The information obtained from this study is very important in daily medical practice because it will provide clinical and bacteriological criteria that will allow anticipating which patients could have an unfavorable evolution or risk of mortality and also have evidence for the rational use of antibiotics.