Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms

Descripción del Articulo

Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Producción Animal
Detalles Bibliográficos
Autores: Morón, J.A., Veli, E.A., Membrillo, A., Paredes, M. M., Gutiérrez, G. A.
Fecha de Publicación:2020
Institución:Universidad Nacional Agraria La Molina
Repositorio:UNALM-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.lamolina.edu.pe:20.500.12996/5038
Enlace del recurso:https://hdl.handle.net/20.500.12996/5038
Materia:Alpaca
Razas (animales)
Registro
Razas indígenas
Mejoramiento animal
Marcadores genéticos
Pruebas de paternidad
Endogamia
Líneas consanguíneas
Ascendencia
Evaluación
Perú
Panel de microsatélites
Región Pasco
https://purl.org/pe-repo/ocde/ford#4.02.01
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dc.title.none.fl_str_mv Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
title Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
spellingShingle Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
Morón, J.A.
Alpaca
Razas (animales)
Registro
Razas indígenas
Mejoramiento animal
Marcadores genéticos
Pruebas de paternidad
Endogamia
Líneas consanguíneas
Ascendencia
Evaluación
Perú
Panel de microsatélites
Región Pasco
https://purl.org/pe-repo/ocde/ford#4.02.01
title_short Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
title_full Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
title_fullStr Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
title_full_unstemmed Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
title_sort Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
author Morón, J.A.
author_facet Morón, J.A.
Veli, E.A.
Membrillo, A.
Paredes, M. M.
Gutiérrez, G. A.
author_role author
author2 Veli, E.A.
Membrillo, A.
Paredes, M. M.
Gutiérrez, G. A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Morón, J.A.
Veli, E.A.
Membrillo, A.
Paredes, M. M.
Gutiérrez, G. A.
dc.subject.none.fl_str_mv Alpaca
Razas (animales)
Registro
Razas indígenas
Mejoramiento animal
Marcadores genéticos
Pruebas de paternidad
Endogamia
Líneas consanguíneas
Ascendencia
Evaluación
Perú
Panel de microsatélites
Región Pasco
topic Alpaca
Razas (animales)
Registro
Razas indígenas
Mejoramiento animal
Marcadores genéticos
Pruebas de paternidad
Endogamia
Líneas consanguíneas
Ascendencia
Evaluación
Perú
Panel de microsatélites
Región Pasco
https://purl.org/pe-repo/ocde/ford#4.02.01
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#4.02.01
description Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Producción Animal
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2021-11-11T20:07:41Z
dc.date.available.none.fl_str_mv 2021-11-11T20:07:41Z
dc.date.issued.fl_str_mv 2020
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.version.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.identifier.citation.none.fl_str_mv J.A. Morón, E.A. Veli, A. Membrillo, M.M. Paredes, G.A Gutiérrez. (2020). Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms, Small Ruminant Research, Volume 193, 2020, 106246, ISSN 0921-4488, https://doi.org/10.1016/j.smallrumres.2020.106246 (https://www.sciencedirect.com/science/article/pii/S0921448820301954)
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12996/5038
identifier_str_mv J.A. Morón, E.A. Veli, A. Membrillo, M.M. Paredes, G.A Gutiérrez. (2020). Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms, Small Ruminant Research, Volume 193, 2020, 106246, ISSN 0921-4488, https://doi.org/10.1016/j.smallrumres.2020.106246 (https://www.sciencedirect.com/science/article/pii/S0921448820301954)
url https://hdl.handle.net/20.500.12996/5038
dc.language.iso.none.fl_str_mv eng
language eng
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
dc.publisher.country.none.fl_str_mv US
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv https://www.sciencedirect.com/science/article/abs/pii/S0921448820301954
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spelling 5bd34c15-2d5a-4b42-b384-a3c7f9bad35d7e3f39f5-f3a9-482e-923e-6a22374d9a671c35f6dd-7b62-475d-aa8b-610c84961e5530889bd0-a243-47a8-948b-27b2dbe1aaa1028c48e7-1609-4324-882d-198fd0d4fdadMorón, J.A.Veli, E.A.Membrillo, A.Paredes, M. M.Gutiérrez, G. A.2021-11-11T20:07:41Z2021-11-11T20:07:41Z2020J.A. Morón, E.A. Veli, A. Membrillo, M.M. Paredes, G.A Gutiérrez. (2020). Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms, Small Ruminant Research, Volume 193, 2020, 106246, ISSN 0921-4488, https://doi.org/10.1016/j.smallrumres.2020.106246 (https://www.sciencedirect.com/science/article/pii/S0921448820301954)https://hdl.handle.net/20.500.12996/5038Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Producción AnimalThe alpaca is of greatest economic importance in the Peruvian High Andes. This study aimed to determine the genetic diversity of three Peruvian alpaca farms, as well as, to validate a microsatellite markers panel for paternity testing. In this study, 247 samples of Huacaya alpacas were taken from three different localities (Sanjo, San Pedro de Raco and Cachipampa) from Pasco Region in Peru. DNA was obtained from hair follicles and genotyped for 15 microsatellites markers in multiplex electrophoresis runs. A total of 225 alleles were detected across the 15 loci investigated. The polymorphism information content considering all loci was 0.82, which indicated that the microsatellite panel was very polymorphic and highly informative. The estimated diversity parameter showed that farms have high levels of genetic diversity (HE = 0.826), and revealed the existence of genetic differentiation among the farms (FST = 2.8 %). The highest inbreeding coefficient was in the Sanjo farm (FIS = 0.303). The results of the parentage testing indicated that all loci showed values greater than 70 % probability of discrimination. However, the highest values found were 94 % (YWLL08) and 90 % (YWLL36). The average of the probability of exclusion obtained was 0.999994 if the genotype for one alleged parents is known, and 0.99999 if the genotypes for both alleged parents are known. The results obtained show that there is a high genetic diversity and validate the panel of microsatellite markers, that would help to improve the identification system and genealogical data collection.application/pdfengElsevierUShttp://purl.org/coar/access_right/c_16echttps://www.sciencedirect.com/science/article/abs/pii/S0921448820301954reponame:UNALM-Institucionalinstname:Universidad Nacional Agraria La Molinainstacron:UNALMAlpacaRazas (animales)RegistroRazas indígenasMejoramiento animalMarcadores genéticosPruebas de paternidadEndogamiaLíneas consanguíneasAscendenciaEvaluaciónPerúPanel de microsatélitesRegión Pascohttps://purl.org/pe-repo/ocde/ford#4.02.01Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farmshttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85ORIGINALmoron-membrillo-paredes-gutierrez.pdfmoron-membrillo-paredes-gutierrez.pdfDatos generalesapplication/pdf28071https://repositorio.lamolina.edu.pe/bitstreams/8e26eec1-d4c6-4aa1-9ec7-999270f371ca/download9ac1e8e2a33fc23ccdfb21d673ce71f3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81664https://repositorio.lamolina.edu.pe/bitstreams/09bdafce-3078-4100-9b8b-78e000eb6c5c/download97c5bee00fbb4c4f8867bd742b579336MD52TEXTmoron-membrillo-paredes-gutierrez.pdf.txtmoron-membrillo-paredes-gutierrez.pdf.txtExtracted texttext/plain1https://repositorio.lamolina.edu.pe/bitstreams/6e54bf3d-cbbb-402c-b055-401f56d13e27/download68b329da9893e34099c7d8ad5cb9c940MD53THUMBNAILmoron-membrillo-paredes-gutierrez.pdf.jpgmoron-membrillo-paredes-gutierrez.pdf.jpgGenerated Thumbnailimage/jpeg4108https://repositorio.lamolina.edu.pe/bitstreams/51d75d48-d07b-462b-a2b6-67e9330aafd8/downloadfcef9c8ae59341b77d96a059dc007e84MD5420.500.12996/5038oai:repositorio.lamolina.edu.pe:20.500.12996/50382023-01-05 04:27:30.224open.accesshttps://repositorio.lamolina.edu.peRepositorio Universidad Nacional Agraria La Molinadspace@lamolina.edu.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Genetic diversity and validation of a microsatellite panel for parentage testing for alpacas (Vicugna pacos) on three Peruvian farms
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