Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test.

• Autores: Salimi-Bejestani, M.R., McGarry, J.W., Felstead, S., Ortiz-Oblitas, P., Akça, A., Williams, D.J.L.
• Formato: artículo
• Fecha de Publicación: 2005
• Institución: Universidad Nacional de Cajamarca
• Repositorio: UNC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.unc.edu.pe:20.500.14074/10303
• Enlace del recurso: http://hdl.handle.net/20.500.14074/10303; https://doi.org/10.1016/j.rvsc.2004.08.005
• Nivel de acceso: acceso abierto
• Materia: Fasciola hepatica; https://purl.org/pe-repo/ocde/ford#4.03.01

An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F. hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic=0.82).