OBTAINING VIRUS FREE PLANTS FROM ASPARAGUS OFFICINALIS L. “Asparagus” IN VITRO

• Autores: Quiñonez Aguilar, Mauro, Riboty Ilas, Renzo
• Formato: artículo
• Fecha de Publicación: 2020
• Institución: Universidad Ricardo Palma
• Repositorio: Revistas - Universidad Ricardo Palma
• Lenguaje: español
• OAI Identifier: oai:oai.revistas.urp.edu.pe:article/2763
• Enlace del recurso: http://revistas.urp.edu.pe/index.php/Scientia/article/view/2763
• Nivel de acceso: acceso abierto
• Materia: Asparagus officinalis L. / micropropagation / 6-Benzyl Aminopurine/ Meristem; Asparagus officinalis L. / Micropropagación / 6-Bencil Aminopurina/ Meristemo/ Cultivo in vitro

This work attempts to obtain free virus Asparagus officinalis L. “garden asparagus”plants, applying the biotechnological methods of the in vitro meristem culture. Axillarybuds were used as explants, and were sterilized, isolated, cultivated in the basalmedium Murashige & Skoog (MS) at 3 different concentrations (0,5; 1,0 & 1,5 mg/L)of the phytohormone 6-Bencil Aminopurine (6-BAP), and incubated under controlledconditions in a cultivation room. As a result, 8 shoots & 8 cladodes, 6 shoots with nocladodes and 1 shoot & 5 cladodes were obtained in the culture mediums supplementedwith the 3 concentrations of 6-BAP respectively.