Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice

• Autores: Castro Rua, Mabel, Gutiérrez Patzi, Evelyn, Pacheco Felipes, Gabriela, Tola Guarachi, Elena, Callisaya Mamani, Ana, Flores Quispe, Mónica, Gonzales Beltrán, Helen, Villca Condori, André, Apaza Chambi, Luz, Terán Ventura, Evangelina
• Formato: artículo
• Fecha de Publicación: 2022
• Institución: Universidad Peruana Unión
• Repositorio: Revistas - Universidad Peruana Unión
• Lenguaje: español
• OAI Identifier: oai:ojs2.revistas.upeu.edu.pe:article/1658
• Enlace del recurso: https://revistas.upeu.edu.pe/index.php/rc_salud/article/view/1658
• Nivel de acceso: acceso abierto
• Materia: Equisetum arvense, Tropaeolum tuberosum, diuretic effect, flavonoids, electrolytes, mice.; Equisetum arvense, Tropaeolum tuberosum, efecto diurético, flavonoides, electrolitos, ratones.

Isaño and cola de caballo are used in Bolivia for their traditional use as a diuretic, but there are nopharmacological studies that confirm this effect. Objective: to determine the diuretic effect of theaqueous extract of isaño and cola de caballo. Methodology: male mice were used, which were dividedinto four groups, a positive and negative control and two dose groups (D1 and D2) of the extract. Bothdried plants were fractionated for the preparation of the aqueous extract, with which the preliminaryphytochemical screening was carried out, and the technique described by Lipschitz was carried out todetermine the diuretic effect. Results: The phytochemical analysis revealed the presence of flavonoidsfor the aqueous extract of cola de caballo. And the aqueous extract of Isaño showed the presenceof tannins, anthocyanins and flavonoids. The highest volume of urine collected was for the positivecontrol, and the lowest volume was for the negative control. The volumes of urine of the extract studiedwith respect to the negative and positive control are not statistically significant, but the value of thevolume of urine of the higher dose is closer to the value of the positive control. The electrolyte resultsshowed a decrease in the excretion of Na +, K + and Cl- in the urine of the problem mice with respectto the negative control group and the positive control, except the K + showed somewhat high valueswith respect to the positive control. Conclusion: The results confirm a possible diuretic effect of isañoand cola de caballo