Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).

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Objective: To evaluate in vitro the solvent and toxic effect of lemon peel oil (Citrus limon) according to exposure time. Its components were identified through gas chromatography. Material and Methods: The study was of experimental type in vitro. To determine the solvent...

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Autores: Herrera-Plasencia, Paul, Garcia-Rupaya, Carmen, Delgado-Cotrina, Leyla
Formato: artículo
Fecha de Publicación:2019
Institución:Universidad Peruana Cayetano Heredia
Repositorio:Revistas - Universidad Peruana Cayetano Heredia
Lenguaje:español
OAI Identifier:oai:revistas.upch.edu.pe:article/3603
Enlace del recurso:https://revistas.upch.edu.pe/index.php/REH/article/view/3603
Nivel de acceso:acceso abierto
Materia:Guttapercha; solvents; volatiles oils; toxicity.
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spelling Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).Eficacia disolvente y citotoxicidad del aceite de cáscara de limón (Citrus limon)Herrera-Plasencia, PaulGarcia-Rupaya, CarmenDelgado-Cotrina, LeylaGuttapercha; solvents; volatiles oils; toxicity.Objective: To evaluate in vitro the solvent and toxic effect of lemon peel oil (Citrus limon) according to exposure time. Its components were identified through gas chromatography. Material and Methods: The study was of experimental type in vitro. To determine the solvent effect, standardized gutta-percha cones of endodontic number 80 with taper 0.02 were used; solvents used were: xylol, lemon and orange peel essential oil; these were immersed in 2, 5 and 10 minutes (n = 8). The weight was recorded before and after the immersion to the substances evaluated to determine the loss of mass. To evaluate the toxicity, Artemias Franciscan were used through the medium lethal concentration; Gas chromatography was used to obtain the components of each oil. The ANOVA / Tukey analysis was used to compare the solvent effect between the substances. Results: No significant difference was found to dissolve the gutta-percha with the lemon, orange and xylol essential oil (p> 0.05). Time did not influence the dissolution of gutta-percha (p> 0.05). All oils were toxic, with the exception of 25% lemon oil. Conclusions: Limonene and D limonene were the components with the highest percentage in lemon and orange oil, respectively. Lemon oil could be considered as an alternative to be used as a gutta-percha solvent for endodontic use, because it has a similar effect as orange oil.Objetivo: Evaluar in vitro el efecto disolvente y tóxico del aceite de cáscara de limón (Citrus limon) según tiempo de exposición e identifi car sus componentes a través de la cromatografía de gases. Material y métodos: El estudio fue de tipo experimental in vitro. Para determinar el efecto disolvente se utilizaron conos de gutapercha estandarizados de endodoncia número 80 con conicidad 0,02; los disolventes utilizados fueron: xilol, aceite esencial de cáscara de limón y naranja; éstos fueron sumergidos en 2, 5 y 10 minutos (n=8). Se registró el peso antes y después de la inmersión a las sustancias evaluadas para determinar la pérdida de masa. Para evaluar la toxicidad se emplearon Artemias franciscanas a través de la concentración letal media; la cromatografía de gases se utilizó para obtener los componentes de cada aceite. Para comparar el efecto disolvente entre las sustancias se empleó el análisis ANOVA/Tukey. Resultados: No se encontró diferencia signifi cativa para disolver la gutapercha con el aceite esencial de cáscara de limón, de naranja y xilol (p>0.05). El tiempo no tuvo influencia para la disolución de la gutapercha (p>0.05). Todos los aceites fueron tóxicos, a excepción del aceite de limón 25%. Conclusiones: El Limoneno y el D limoneno fueron los componentes con mayor porcentaje en el aceite de limón y de naranja, respectivamente. El aceite de limón podría ser una considerada como alternativa para ser utilizado como disolvente de gutapercha de uso endodóntico, por tener efecto similar que el aceite naranja.Universidad Peruana Cayetano Heredia2019-11-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.upch.edu.pe/index.php/REH/article/view/360310.20453/reh.v29i3.3603Revista Estomatológica Herediana; Vol. 29 No. 3 (2019): Julio - Setiembre; 196-202Revista Estomatológica Herediana; Vol. 29 Núm. 3 (2019): Julio - Setiembre; 196-202Revista Estomatológica Herediana; v. 29 n. 3 (2019): Julio - Setiembre; 196-2022225-76161019-4355reponame:Revistas - Universidad Peruana Cayetano Herediainstname:Universidad Peruana Cayetano Herediainstacron:UPCHspahttps://revistas.upch.edu.pe/index.php/REH/article/view/3603/3997info:eu-repo/semantics/openAccessoai:revistas.upch.edu.pe:article/36032020-06-16T19:42:53Z
dc.title.none.fl_str_mv Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
Eficacia disolvente y citotoxicidad del aceite de cáscara de limón (Citrus limon)
title Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
spellingShingle Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
Herrera-Plasencia, Paul
Guttapercha; solvents; volatiles oils; toxicity.
title_short Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
title_full Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
title_fullStr Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
title_full_unstemmed Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
title_sort Solvent efficiency and cytotoxicity of lemon peel oil (Citrus limon).
dc.creator.none.fl_str_mv Herrera-Plasencia, Paul
Garcia-Rupaya, Carmen
Delgado-Cotrina, Leyla
author Herrera-Plasencia, Paul
author_facet Herrera-Plasencia, Paul
Garcia-Rupaya, Carmen
Delgado-Cotrina, Leyla
author_role author
author2 Garcia-Rupaya, Carmen
Delgado-Cotrina, Leyla
author2_role author
author
dc.subject.none.fl_str_mv Guttapercha; solvents; volatiles oils; toxicity.
topic Guttapercha; solvents; volatiles oils; toxicity.
description Objective: To evaluate in vitro the solvent and toxic effect of lemon peel oil (Citrus limon) according to exposure time. Its components were identified through gas chromatography. Material and Methods: The study was of experimental type in vitro. To determine the solvent effect, standardized gutta-percha cones of endodontic number 80 with taper 0.02 were used; solvents used were: xylol, lemon and orange peel essential oil; these were immersed in 2, 5 and 10 minutes (n = 8). The weight was recorded before and after the immersion to the substances evaluated to determine the loss of mass. To evaluate the toxicity, Artemias Franciscan were used through the medium lethal concentration; Gas chromatography was used to obtain the components of each oil. The ANOVA / Tukey analysis was used to compare the solvent effect between the substances. Results: No significant difference was found to dissolve the gutta-percha with the lemon, orange and xylol essential oil (p> 0.05). Time did not influence the dissolution of gutta-percha (p> 0.05). All oils were toxic, with the exception of 25% lemon oil. Conclusions: Limonene and D limonene were the components with the highest percentage in lemon and orange oil, respectively. Lemon oil could be considered as an alternative to be used as a gutta-percha solvent for endodontic use, because it has a similar effect as orange oil.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.upch.edu.pe/index.php/REH/article/view/3603
10.20453/reh.v29i3.3603
url https://revistas.upch.edu.pe/index.php/REH/article/view/3603
identifier_str_mv 10.20453/reh.v29i3.3603
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.upch.edu.pe/index.php/REH/article/view/3603/3997
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Peruana Cayetano Heredia
publisher.none.fl_str_mv Universidad Peruana Cayetano Heredia
dc.source.none.fl_str_mv Revista Estomatológica Herediana; Vol. 29 No. 3 (2019): Julio - Setiembre; 196-202
Revista Estomatológica Herediana; Vol. 29 Núm. 3 (2019): Julio - Setiembre; 196-202
Revista Estomatológica Herediana; v. 29 n. 3 (2019): Julio - Setiembre; 196-202
2225-7616
1019-4355
reponame:Revistas - Universidad Peruana Cayetano Heredia
instname:Universidad Peruana Cayetano Heredia
instacron:UPCH
instname_str Universidad Peruana Cayetano Heredia
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reponame_str Revistas - Universidad Peruana Cayetano Heredia
collection Revistas - Universidad Peruana Cayetano Heredia
repository.name.fl_str_mv
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