Efeitos de convulsões de kindling induzidas por pentilenotetrazol a longo prazo na neuroinflamação: análise imuno-histoquímica e de EEG em ratos

• Autores: Yauri Huamán, Yessenia, Cáceres Robles, Rodrigo Jhampier, Becerra Flores, Christell Tatiana
• Formato: artículo
• Fecha de Publicación: 2025
• Institución: Universidad Nacional San Antonio Abad del Cusco
• Repositorio: Revistas - Universidad Nacional San Antonio Abad del Cusco
• Lenguaje: español
• OAI Identifier: oai:ojs.pkp.sfu.ca:article/1789
• Enlace del recurso: https://revistas.unsaac.edu.pe/index.php/RQ/article/view/1789
• Nivel de acceso: acceso abierto
• Materia: Pentylenetetrazol; Chronic kindling; GFAP; NeuN; Hippocampus; Pentilentetrazol; Kindling crónico; Hipocampo

The present experimental study examines the effects of PTZ-induced kindling, a well-established and widely used animal model for studying epileptogenesis through the progressive induction of seizures. The novelty of this research lies in its descriptive analysis of the post-kindling phase, focusing on both brain activity and molecular changes. Twelve rats were subjected to a three-phase protocol: habituation, challenge (60 mg/kg), and kindling, which involved repeated administration of subconvulsive doses of PTZ (35 mg/kg). The control group received only saline injections. In the first month following kindling, a subset of animals was implanted with epidural electrodes to record brain activity through electroencephalograph (EEG). In the second month, another subset was used for molecular analyses using immunohistochemistry (IHC) and immunofluorescence (IF), with GFAP and NeuN serving as markers for astrocytes and mature neurons, respectively. These molecular assessments were repeated in the third month post-kindling. EEG recordings revealed interictal events during the first post-kindling month. In the second and third months, a significant increase in GFAP expression was observed in hippocampal regions (CA1, CA3, and dentate gyrus), while NeuN expression remained unchanged. These findings contribute to the characterization of the post-kindling phase and enhance the understanding of the PTZ model's underlying mechanisms and temporal progression.