Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)

Descripción del Articulo

The present study evaluated the presence of 10 genes encoding various virulence factors of biological importance in 100 Salmonella Typhimurium isolates, where 90 isolates came from sick guinea pigs and 10 from apparently healthy ones. DNA was extracted from all isolates and analyzed using the Multip...

Descripción completa

Detalles Bibliográficos
Autores: Duran Gonzalez, Carla, Luna Espinoza, Luis, Carhuaricra Huamán, Dennis, Salvatierra Rodríguez, Guillermo, Rosadio Alcántara, Raúl, Maturrano Hernández, Lenin
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/21331
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21331
Nivel de acceso:acceso abierto
Materia:guinea pig
Salmonella Typhimurium
virulence factors
PCR Multiplex
cuy
factores de virulencia
PCR Múltiple
id REVUNMSM_d1dacc898a870ce8762dcf8dbb18cb65
oai_identifier_str oai:ojs.csi.unmsm:article/21331
network_acronym_str REVUNMSM
network_name_str Revistas - Universidad Nacional Mayor de San Marcos
repository_id_str
spelling Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)Evaluación de factores de virulencia en cepas de Salmonella Typhimurium aisladas de cuyes (Cavia porcellus) enfermos y sanosDuran Gonzalez, CarlaLuna Espinoza, LuisCarhuaricra Huamán, DennisSalvatierra Rodríguez, GuillermoRosadio Alcántara, RaúlMaturrano Hernández, LeninDuran Gonzalez, CarlaLuna Espinoza, LuisCarhuaricra Huamán, DennisSalvatierra Rodríguez, GuillermoRosadio Alcántara, RaúlMaturrano Hernández, Leninguinea pigSalmonella Typhimuriumvirulence factorsPCR MultiplexcuySalmonella Typhimuriumfactores de virulenciaPCR MúltipleThe present study evaluated the presence of 10 genes encoding various virulence factors of biological importance in 100 Salmonella Typhimurium isolates, where 90 isolates came from sick guinea pigs and 10 from apparently healthy ones. DNA was extracted from all isolates and analyzed using the Multiple PCR technique to assess the presence of the spvB, spiA, cdtB, sipB, tolC, sitC, lpfC, sifA, sopB and pefA genes. A similar genetic pattern was obtained, with variable detection frequencies greater than 60%, both in healthy and sick guinea pigs, except for the cdtB gene, which was not detected. There were no significant differences between the presence of Salmonella Typhimurium virulence factors isolated from sick guinea pigs and apparently healthy guinea pigs.El presente estudio evaluó la presencia de 10 genes codificantes de diversos factores de virulencia de importancia biológica en un total de 100 aislados de Salmonella Typhimurium, donde 90 aislados procedían de cuyes enfermos y 10 de cuyes aparentemente sanos. El ADN fue extraído de todos los aislados y se analizaron mediante la técnica de PCR múltiple para evaluar la presencia de los genes spvB, spiA, cdtB, sipB, tolC, sitC, lpfC, sifA, sopB y pefA. Se obtuvo un patrón genético similar, con frecuencias de detección variables mayores al 60%, tanto en aislados de cuyes sanos como enfermos, excepto el gen cdtB, el cual no fue detectado. No hubo diferencias significativas entre la presencia de factores de virulencia de Salmonella Typhimurium aisladas de cuyes enfermos y de cuyes aparentemente sanos.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2021-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2133110.15381/rivep.v32i5.21331Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 5 (2021); e21331Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 5 (2021); e213311682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21331/17300Derechos de autor 2021 Carla Duran Gonzalez, Luis Luna Espinoza, Dennis Carhuaricra Huamán, Guillermo Salvatierra Rodríguez, Raúl Rosadio Alcántara, Lenin Maturrano Hernándezhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/213312021-10-27T23:31:48Z
dc.title.none.fl_str_mv Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
Evaluación de factores de virulencia en cepas de Salmonella Typhimurium aisladas de cuyes (Cavia porcellus) enfermos y sanos
title Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
spellingShingle Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
Duran Gonzalez, Carla
guinea pig
Salmonella Typhimurium
virulence factors
PCR Multiplex
cuy
Salmonella Typhimurium
factores de virulencia
PCR Múltiple
title_short Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
title_full Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
title_fullStr Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
title_full_unstemmed Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
title_sort Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
dc.creator.none.fl_str_mv Duran Gonzalez, Carla
Luna Espinoza, Luis
Carhuaricra Huamán, Dennis
Salvatierra Rodríguez, Guillermo
Rosadio Alcántara, Raúl
Maturrano Hernández, Lenin
Duran Gonzalez, Carla
Luna Espinoza, Luis
Carhuaricra Huamán, Dennis
Salvatierra Rodríguez, Guillermo
Rosadio Alcántara, Raúl
Maturrano Hernández, Lenin
author Duran Gonzalez, Carla
author_facet Duran Gonzalez, Carla
Luna Espinoza, Luis
Carhuaricra Huamán, Dennis
Salvatierra Rodríguez, Guillermo
Rosadio Alcántara, Raúl
Maturrano Hernández, Lenin
author_role author
author2 Luna Espinoza, Luis
Carhuaricra Huamán, Dennis
Salvatierra Rodríguez, Guillermo
Rosadio Alcántara, Raúl
Maturrano Hernández, Lenin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv guinea pig
Salmonella Typhimurium
virulence factors
PCR Multiplex
cuy
Salmonella Typhimurium
factores de virulencia
PCR Múltiple
topic guinea pig
Salmonella Typhimurium
virulence factors
PCR Multiplex
cuy
Salmonella Typhimurium
factores de virulencia
PCR Múltiple
description The present study evaluated the presence of 10 genes encoding various virulence factors of biological importance in 100 Salmonella Typhimurium isolates, where 90 isolates came from sick guinea pigs and 10 from apparently healthy ones. DNA was extracted from all isolates and analyzed using the Multiple PCR technique to assess the presence of the spvB, spiA, cdtB, sipB, tolC, sitC, lpfC, sifA, sopB and pefA genes. A similar genetic pattern was obtained, with variable detection frequencies greater than 60%, both in healthy and sick guinea pigs, except for the cdtB gene, which was not detected. There were no significant differences between the presence of Salmonella Typhimurium virulence factors isolated from sick guinea pigs and apparently healthy guinea pigs.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21331
10.15381/rivep.v32i5.21331
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21331
identifier_str_mv 10.15381/rivep.v32i5.21331
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21331/17300
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 5 (2021); e21331
Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 5 (2021); e21331
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238236280324096
score 13.754011
Evaluation of virulence factors of strains of Salmonella Typhimurium isolated from diseased and healthy guinea pigs (Cavia porcellus)
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

Ejemplares Similares