Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus

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Gametes and larval stages of the mussels P purpuratus and S. algosus were treated in vitro with fluorescent and non-luorescent techniques in order to detect microfilaments, DNA and cortical granules involved with reproductive status as fertilization and cleaveage stages. In P. purpuratus tubulin was...

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Detalles Bibliográficos
Autores: Garrido, Orlando, Pino, José
Formato: artículo
Fecha de Publicación:1999
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/8294
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8294
Nivel de acceso:acceso abierto
Materia:Mejillones
Inmunofluorescencia
DAPI
tubulina
Perumytilus
Semimytilus
microfilamentos
gránulos corticales
Chile.
Mussels
Immunofluorescence
tubulin
Perumyilus
microfilaments
cortical granules
Chile
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spelling Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosusCaracterización de algunas técnicas de inmunofluorescencia y de fluorescencia en mejillones chilenos Perumytilus purpuratus y Semimytilus algosusGarrido, OrlandoPino, JoséMejillonesInmunofluorescenciaDAPItubulinaPerumytilusSemimytilusmicrofilamentosgránulos corticalesChile.MusselsImmunofluorescenceDAPItubulinPerumyilusSemimytilusmicrofilamentscortical granulesChileGametes and larval stages of the mussels P purpuratus and S. algosus were treated in vitro with fluorescent and non-luorescent techniques in order to detect microfilaments, DNA and cortical granules involved with reproductive status as fertilization and cleaveage stages. In P. purpuratus tubulin was detected in cilius and velum in D larval stages, also actin was detected from the fertilization to development stages in both P purouratus and S. algosus. Cortical granule-like structures were observed in larval stages of P purpuratus suggesting they are not discharged during the fertilization process. Microfilaments detected in both mussels suggest they play an important role in the cytoskeleton during development.Gametos y estadios larvales de los mejillones P. purpuratus y S. algosus fueron tratados in vitro con técnicas de fluorescencia y no fluorescencia a fin de detectar microfilamentos, DNA y gránulos corticales involucrados con estadios de la reproducción como fertilización y clivaje. En P. purouratus se detectó tubulina a nivel de los cilios y el velum; asimismo, la actina fue detectada desde el estadio de fertilización a los estadios de desarrollo tanto en P purpuratus como en S. algosus, lo que sugiere que no son descartados durante el proceso de fertilización. Los microfilamentos detectados en ambos mejillones sugieren que ellos juegan un rol importante como integrante del citoesqueleto durante el desarrollo.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas1999-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/829410.15381/rpb.v6i1.8294Revista Peruana de Biología; Vol. 6 Núm. 1 (1999); 104 - 109Revista Peruana de Biología; Vol. 6 No. 1 (1999); 104 - 1091727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8294/7220Derechos de autor 1999 Orlando Garrido, José Pinohttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/82942020-05-26T11:57:40Z
dc.title.none.fl_str_mv Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
Caracterización de algunas técnicas de inmunofluorescencia y de fluorescencia en mejillones chilenos Perumytilus purpuratus y Semimytilus algosus
title Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
spellingShingle Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
Garrido, Orlando
Mejillones
Inmunofluorescencia
DAPI
tubulina
Perumytilus
Semimytilus
microfilamentos
gránulos corticales
Chile.
Mussels
Immunofluorescence
DAPI
tubulin
Perumyilus
Semimytilus
microfilaments
cortical granules
Chile
title_short Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
title_full Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
title_fullStr Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
title_full_unstemmed Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
title_sort Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
dc.creator.none.fl_str_mv Garrido, Orlando
Pino, José
author Garrido, Orlando
author_facet Garrido, Orlando
Pino, José
author_role author
author2 Pino, José
author2_role author
dc.subject.none.fl_str_mv Mejillones
Inmunofluorescencia
DAPI
tubulina
Perumytilus
Semimytilus
microfilamentos
gránulos corticales
Chile.
Mussels
Immunofluorescence
DAPI
tubulin
Perumyilus
Semimytilus
microfilaments
cortical granules
Chile
topic Mejillones
Inmunofluorescencia
DAPI
tubulina
Perumytilus
Semimytilus
microfilamentos
gránulos corticales
Chile.
Mussels
Immunofluorescence
DAPI
tubulin
Perumyilus
Semimytilus
microfilaments
cortical granules
Chile
description Gametes and larval stages of the mussels P purpuratus and S. algosus were treated in vitro with fluorescent and non-luorescent techniques in order to detect microfilaments, DNA and cortical granules involved with reproductive status as fertilization and cleaveage stages. In P. purpuratus tubulin was detected in cilius and velum in D larval stages, also actin was detected from the fertilization to development stages in both P purouratus and S. algosus. Cortical granule-like structures were observed in larval stages of P purpuratus suggesting they are not discharged during the fertilization process. Microfilaments detected in both mussels suggest they play an important role in the cytoskeleton during development.
publishDate 1999
dc.date.none.fl_str_mv 1999-06-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8294
10.15381/rpb.v6i1.8294
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8294
identifier_str_mv 10.15381/rpb.v6i1.8294
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8294/7220
dc.rights.none.fl_str_mv Derechos de autor 1999 Orlando Garrido, José Pino
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 1999 Orlando Garrido, José Pino
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 6 Núm. 1 (1999); 104 - 109
Revista Peruana de Biología; Vol. 6 No. 1 (1999); 104 - 109
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238310575079424
score 13.92687
Characterization of some techniques of immunofluorescence and fluorescence in chilean mussels Perumytilus purpuratus and Semimytilus algosus
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