Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers

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The aim of this study was to determine the antimicrobial resistance of 176 Escherichia coli strains isolated from broiler chicken organs. The strains were challenged with beta-lactam, quinolones and fluoroquinolones, observing resistance to beta-lactam antibiotics (97.7%) and quinolones (86.7%). The...

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Detalles Bibliográficos
Autores: Carvajal B., Edna, Rueda G., Egberto, Talavera R., Martín, Torres C., María, López V., Diana, Vásquez R., María C.
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/20012
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20012
Nivel de acceso:acceso abierto
Materia:antimicrobial resistance
beta lactamases
quinolones
broiler chicken
resistencia antimicrobiana
betalactamasas
quinolonas
pollo de carne
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network_name_str Revistas - Universidad Nacional Mayor de San Marcos
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spelling Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilersResistencia a antibióticos betalactámicos y quinolonas en Escherichia coli aislada de pollos broilerCarvajal B., EdnaRueda G., EgbertoTalavera R., MartínTorres C., MaríaLópez V., DianaVásquez R., María C.antimicrobial resistancebeta lactamasesquinolonesbroiler chickenresistencia antimicrobianabetalactamasasquinolonaspollo de carneThe aim of this study was to determine the antimicrobial resistance of 176 Escherichia coli strains isolated from broiler chicken organs. The strains were challenged with beta-lactam, quinolones and fluoroquinolones, observing resistance to beta-lactam antibiotics (97.7%) and quinolones (86.7%). The results showed that 71.6% of the isolates phenotypically expressed the production of extended spectrum beta-lactamases (ESLB). By PCR, resistance genes for beta-lactams blaTEM, blaSHV, blaCTX-M1 and Amp-C and resistance genes for quinolones qnrA, qnrB, qnrS were determined. The genes Amp-C (74%), blaCTX-M (65%), blaSHV (65%), blaTEM (50%), qnrB (86.4%) and qnrS (11.9%) were found. The qnrA gene was not evident in the samples analysed. The results obtained revealed a large percentage of resistance to the studied antibiotics and the presence of resistance genes in isolates from poultry for human consumption, which constitutes a risk for Public Health.El objetivo del estudio fue determinar la resistencia antimicrobiana de 176 cepas de Escherichia. coli aisladas de órganos de pollos broiler. Las cepas fueron desafiadas con antibióticos betalactámicos, quinolonas y fluoroquinolonas, observándose resistencia a antibióticos betalactámicos (97.7%) y a quinolonas (86.7%). El 71.6% de los aislados también expresaron fenotípicamente la producción de betalactamasas de espectro extendido (ESLB). Mediante PCR se determinaron genes de resistencia para betalactámicos blaTEM, blaSHV, blaCTX-M1 y Amp-C y genes de resistencia para quinolonas qnrA, qnrB, qnrS. Se encontraron los genes Amp-C (74%), blaCTX-M (65%), blaSHV (65%), blaTEM (50%), qnrB (86.4%) y qnrS (11.9%). No se evidenció el gen qnrA en las muestras analizadas. Los resultados obtenidos revelaron un gran porcentaje de resistencia a los antibióticos estudiados y la presencia de genes de resistencia en aislados de aves para consumo humano, lo cual constituye un riesgo para la salud pública.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2021-04-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2001210.15381/rivep.v32i2.20012Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 2 (2021); e20012Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 2 (2021); e200121682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20012/16544Derechos de autor 2021 Edna Carvajal B., Egberto Rueda G., Martín Talavera R., María Torres C., Diana López V., María C. Vásquez R.https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/200122021-04-26T16:45:30Z
dc.title.none.fl_str_mv Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
Resistencia a antibióticos betalactámicos y quinolonas en Escherichia coli aislada de pollos broiler
title Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
spellingShingle Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
Carvajal B., Edna
antimicrobial resistance
beta lactamases
quinolones
broiler chicken
resistencia antimicrobiana
betalactamasas
quinolonas
pollo de carne
title_short Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
title_full Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
title_fullStr Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
title_full_unstemmed Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
title_sort Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
dc.creator.none.fl_str_mv Carvajal B., Edna
Rueda G., Egberto
Talavera R., Martín
Torres C., María
López V., Diana
Vásquez R., María C.
author Carvajal B., Edna
author_facet Carvajal B., Edna
Rueda G., Egberto
Talavera R., Martín
Torres C., María
López V., Diana
Vásquez R., María C.
author_role author
author2 Rueda G., Egberto
Talavera R., Martín
Torres C., María
López V., Diana
Vásquez R., María C.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv antimicrobial resistance
beta lactamases
quinolones
broiler chicken
resistencia antimicrobiana
betalactamasas
quinolonas
pollo de carne
topic antimicrobial resistance
beta lactamases
quinolones
broiler chicken
resistencia antimicrobiana
betalactamasas
quinolonas
pollo de carne
description The aim of this study was to determine the antimicrobial resistance of 176 Escherichia coli strains isolated from broiler chicken organs. The strains were challenged with beta-lactam, quinolones and fluoroquinolones, observing resistance to beta-lactam antibiotics (97.7%) and quinolones (86.7%). The results showed that 71.6% of the isolates phenotypically expressed the production of extended spectrum beta-lactamases (ESLB). By PCR, resistance genes for beta-lactams blaTEM, blaSHV, blaCTX-M1 and Amp-C and resistance genes for quinolones qnrA, qnrB, qnrS were determined. The genes Amp-C (74%), blaCTX-M (65%), blaSHV (65%), blaTEM (50%), qnrB (86.4%) and qnrS (11.9%) were found. The qnrA gene was not evident in the samples analysed. The results obtained revealed a large percentage of resistance to the studied antibiotics and the presence of resistance genes in isolates from poultry for human consumption, which constitutes a risk for Public Health.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20012
10.15381/rivep.v32i2.20012
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20012
identifier_str_mv 10.15381/rivep.v32i2.20012
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20012/16544
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 2 (2021); e20012
Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 2 (2021); e20012
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.959364
Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers
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