Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks

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In this paper we identify an unexpected DNA sequence from the amplicons of 28S rRNA gene of terrestrial mollusks. DNA extractions were performed from foot tissues of land snails using a modified CTAB protocol. PCRs were carried out with universal primers for COI gene and oligonucleotides designed fo...

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Detalles Bibliográficos
Autores: Chirinos, Jenny, Congrains, Carlos, Ramírez, Rina, Ramírez, Pablo
Formato: artículo
Fecha de Publicación:2010
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/52
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/52
Nivel de acceso:acceso abierto
Materia:26S rRNA
Gastropoda
fungi
código de barras de DNA.
DNA barcode.
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spelling Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusksIdentificación de Yarrowia lipolytica (Ascomycota: Hemiascomycetes) como contaminante en la obtención de amplificados del gen 28S rRNA de moluscosChirinos, JennyCongrains, CarlosRamírez, RinaRamírez, Pablo26S rRNAGastropodafungicódigo de barras de DNA.26S rRNAGastropodafungiDNA barcode.In this paper we identify an unexpected DNA sequence from the amplicons of 28S rRNA gene of terrestrial mollusks. DNA extractions were performed from foot tissues of land snails using a modified CTAB protocol. PCRs were carried out with universal primers for COI gene and oligonucleotides designed for molluscs, for the markers 16S rRNA, 28S rRNA, and ITS-2. Amplified lengths were 706 pb for COI, 330 pb for 16S rRNA, 900 pb for ITS-2, and 583 pb for 28S rRNA. One amplicon of the last marker was of an unexpected length, ~340 pb. DNA sequences were compared in the GenBank database through the BLASTn program and the sample, with the unexpected length, resulted in 100% identity and 99% query coverage with 26S rRNA gene of the yeast Yarrowia lipolytica. Phylogenetic analysis with Neighbour-Joining and the divergence values confirmed the identification, providing results that support the taxonomic placement of the species within the Hemiascomycetes clade. En el presente trabajo se identifica una secuencia de DNA no esperada proveniente de los amplificados del gen 28S rRNA de moluscos terrestres. Las extracciones de DNA se realizaron del tejido del pie de caracoles terrestres por el método del CTAB modificado. Las PCRs fueron llevadas a cabo con primers universales para el gen COI e iniciadores diseñados para moluscos, para el marcador 16S rRNA, 28S rRNA y la región ITS-2. Los tamaños aproximados de las bandas de los amplificados de moluscos fueron de 706 pb para el COI, 330 pb para el 16S rRNA, 900 pb para el ITS-2 y 583 pb para el 28S rRNA; un amplificado del último marcador fue de una longitud inesperada, ~340 pb. Las secuencias de DNA fueron comparadas con la base de datos del GenBank mediante el programa BLASTn y la muestra con la banda de tamaño inesperado resultó en un 100% de identidad y cobertura del 99% con el gen 26S rRNA de la levadura Yarrowia lipolytica. El análisis filogenético con Neighbour-Joining y los valores de divergencia confirmaron la identificación, proporcionando resultados que apoyan la ubicación taxonómica de la especie dentro del clado de los Hemiascomycetes.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2010-04-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/5210.15381/rpb.v17i1.52Revista Peruana de Biología; Vol. 17 Núm. 1 (2010); 059- 064Revista Peruana de Biología; Vol. 17 No. 1 (2010); 059- 0641727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/52/47Derechos de autor 2010 Jenny Chirinos, Carlos Congrains, Rina Ramírez, Pablo Ramírezhttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/522020-05-21T18:06:11Z
dc.title.none.fl_str_mv Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
Identificación de Yarrowia lipolytica (Ascomycota: Hemiascomycetes) como contaminante en la obtención de amplificados del gen 28S rRNA de moluscos
title Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
spellingShingle Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
Chirinos, Jenny
26S rRNA
Gastropoda
fungi
código de barras de DNA.
26S rRNA
Gastropoda
fungi
DNA barcode.
title_short Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
title_full Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
title_fullStr Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
title_full_unstemmed Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
title_sort Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
dc.creator.none.fl_str_mv Chirinos, Jenny
Congrains, Carlos
Ramírez, Rina
Ramírez, Pablo
author Chirinos, Jenny
author_facet Chirinos, Jenny
Congrains, Carlos
Ramírez, Rina
Ramírez, Pablo
author_role author
author2 Congrains, Carlos
Ramírez, Rina
Ramírez, Pablo
author2_role author
author
author
dc.subject.none.fl_str_mv 26S rRNA
Gastropoda
fungi
código de barras de DNA.
26S rRNA
Gastropoda
fungi
DNA barcode.
topic 26S rRNA
Gastropoda
fungi
código de barras de DNA.
26S rRNA
Gastropoda
fungi
DNA barcode.
description In this paper we identify an unexpected DNA sequence from the amplicons of 28S rRNA gene of terrestrial mollusks. DNA extractions were performed from foot tissues of land snails using a modified CTAB protocol. PCRs were carried out with universal primers for COI gene and oligonucleotides designed for molluscs, for the markers 16S rRNA, 28S rRNA, and ITS-2. Amplified lengths were 706 pb for COI, 330 pb for 16S rRNA, 900 pb for ITS-2, and 583 pb for 28S rRNA. One amplicon of the last marker was of an unexpected length, ~340 pb. DNA sequences were compared in the GenBank database through the BLASTn program and the sample, with the unexpected length, resulted in 100% identity and 99% query coverage with 26S rRNA gene of the yeast Yarrowia lipolytica. Phylogenetic analysis with Neighbour-Joining and the divergence values confirmed the identification, providing results that support the taxonomic placement of the species within the Hemiascomycetes clade.
publishDate 2010
dc.date.none.fl_str_mv 2010-04-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/52
10.15381/rpb.v17i1.52
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/52
identifier_str_mv 10.15381/rpb.v17i1.52
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/52/47
dc.rights.none.fl_str_mv Derechos de autor 2010 Jenny Chirinos, Carlos Congrains, Rina Ramírez, Pablo Ramírez
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2010 Jenny Chirinos, Carlos Congrains, Rina Ramírez, Pablo Ramírez
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 17 Núm. 1 (2010); 059- 064
Revista Peruana de Biología; Vol. 17 No. 1 (2010); 059- 064
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.905282
Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks
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