Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR

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The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identifi...

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Detalles Bibliográficos
Autores: Marcelo M., Geraldine, Rosadio A., Raúl, Chero O., Ana, Díaz O., Gerardo, Ciprian C., Aldo, Maturrano H., Lenin
Formato: artículo
Fecha de Publicación:2017
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/13074
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074
Nivel de acceso:acceso abierto
Materia:cuyes
Salmonella Typhimurium
Salmonella Enteritidis
PCR múltiple
guinea pigs
multiplex PCR
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spelling Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCRIdentificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR MúltipleMarcelo M., GeraldineRosadio A., RaúlChero O., AnaDíaz O., GerardoCiprian C., AldoMaturrano H., LenincuyesSalmonella TyphimuriumSalmonella EnteritidisPCR múltipleguinea pigsSalmonella TyphimuriumSalmonella Enteritidismultiplex PCRThe aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from guinea pigs.El objetivo del presente estudio fue identificar mediante PCR múltiple la posible existencia de los serovares Salmonella Typhimurium y Enteritidis en 25 cepas de Salmonella spp previamente aisladas de cuyes e identificadas por sus características metabólicas. Mediante el análisis molecular se identificaron todas las cepas como Salmonella Typhimurium, evidenciando la amplificación de los cebadores específicos para los genes invA y fliC pertenecientes al género Salmonella y Salmonella Typhimurium, respectivamente. El presente estudio permitió establecer una metodología rápida para la identificación molecular de Salmonella Typhimurium y Enteritidis aislados de cuyes.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2017-07-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1307410.15381/rivep.v28i2.13074Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 2 (2017); 411-417Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 2 (2017); 411-4171682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074/11926Derechos de autor 2017 Geraldine Marcelo M., Raúl Rosadio A., Ana Chero O., Gerardo Díaz O., Aldo Ciprian C., Lenin Maturrano H.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/130742017-09-04T16:08:14Z
dc.title.none.fl_str_mv Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple
title Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
spellingShingle Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
Marcelo M., Geraldine
cuyes
Salmonella Typhimurium
Salmonella Enteritidis
PCR múltiple
guinea pigs
Salmonella Typhimurium
Salmonella Enteritidis
multiplex PCR
title_short Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_full Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_fullStr Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_full_unstemmed Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_sort Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
dc.creator.none.fl_str_mv Marcelo M., Geraldine
Rosadio A., Raúl
Chero O., Ana
Díaz O., Gerardo
Ciprian C., Aldo
Maturrano H., Lenin
author Marcelo M., Geraldine
author_facet Marcelo M., Geraldine
Rosadio A., Raúl
Chero O., Ana
Díaz O., Gerardo
Ciprian C., Aldo
Maturrano H., Lenin
author_role author
author2 Rosadio A., Raúl
Chero O., Ana
Díaz O., Gerardo
Ciprian C., Aldo
Maturrano H., Lenin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv cuyes
Salmonella Typhimurium
Salmonella Enteritidis
PCR múltiple
guinea pigs
Salmonella Typhimurium
Salmonella Enteritidis
multiplex PCR
topic cuyes
Salmonella Typhimurium
Salmonella Enteritidis
PCR múltiple
guinea pigs
Salmonella Typhimurium
Salmonella Enteritidis
multiplex PCR
description The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from guinea pigs.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074
10.15381/rivep.v28i2.13074
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074
identifier_str_mv 10.15381/rivep.v28i2.13074
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074/11926
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 2 (2017); 411-417
Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 2 (2017); 411-417
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238227724992512
score 13.892819
Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
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