Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.

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A member of the genus Alteromonas sp. (strain N22.C) isolated from marine neuston and substance-producer which inhibit growth of ichthyopathogenics was characterized phenotipically by means of morphological, physiological and biochemical conventional tests. To determine the chemical nature of the in...

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Detalles Bibliográficos
Autores: León, Jorge, Tapia, Guillermina, Avalos, Rita
Formato: artículo
Fecha de Publicación:2005
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/2411
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2411
Nivel de acceso:acceso abierto
Materia:bacterias marinas
actividad inhibitoria
sustancia extracelular
Alteromonas
antibiosis
marine bacteria
inhibitory substances
extracellular substance
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spelling Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.Purificación parcial y caracterización de una sustancia antimicrobiana producida por Alteromonas sp. de origen marinoLeón, JorgeTapia, GuillerminaAvalos, Ritabacterias marinasactividad inhibitoriasustancia extracelularAlteromonasantibiosismarine bacteriainhibitory substancesextracellular substanceAlteromonasantibiosisA member of the genus Alteromonas sp. (strain N22.C) isolated from marine neuston and substance-producer which inhibit growth of ichthyopathogenics was characterized phenotipically by means of morphological, physiological and biochemical conventional tests. To determine the chemical nature of the inhibitory substance, raw extracts of supernatans from cultured strains were precipitated with increasing concentrations of Ammonium Sulphate up to 70% and filtered through columns of Sephadex G-25. Assays by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS–PAGE) revealed that the antimicrobial substance is a proteinaceous compound with a molecular mass of approximately 34000 Da, which lacks sugar associated residues of it. Other assays made with raw semipurified fractions extracts of Alteromonas sp N22.C show the wide spectrum of antibiotic activity against pathogenic bacterial strains of fish, mollusks and crustaceans.Un miembro del género Alteromonas sp. (cepa N22.C), aislado del neuston marino y productor de sustancias que inhiben el crecimiento de ictiopatógenos fue caracterizado fenotípicamente mediante pruebas morfológicas, fisiológicas y bioquímicas convencionales. Para determinar la naturaleza quí- mica de la sustancia inhibitoria, extractos crudos de sobrenadantes de cultivos fueron precipitados con concentraciones crecientes de Sulfato de Amonio hasta el 70% y filtrados a través de columnas de Sephadex G-25. Ensayos con Dodecil Sulfato de Sodio-electroforesis en Geles de Poliacrilamida (SDS– PAGE) revelaron que la sustancia antimicrobiana es un compuesto proteináceo con una masa molecular de aproximadamente 34000 Da, que carece de residuos de azúcares asociados. Otros ensayos realizados con extractos crudos y fracciones semipurificadas de la cepa de Alteromonas sp. N22.C mostraron un amplio espectro de actividad antibiótica contra cepas de bacterias patógenas de peces, moluscos y crustáceos.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2005-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/241110.15381/rpb.v12i3.2411Revista Peruana de Biología; Vol. 12 Núm. 3 (2005); 359-368Revista Peruana de Biología; Vol. 12 No. 3 (2005); 359-3681727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2411/2110Derechos de autor 2005 Jorge León, Guillermina Tapia, Rita Avaloshttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/24112020-05-25T11:19:13Z
dc.title.none.fl_str_mv Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
Purificación parcial y caracterización de una sustancia antimicrobiana producida por Alteromonas sp. de origen marino
title Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
spellingShingle Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
León, Jorge
bacterias marinas
actividad inhibitoria
sustancia extracelular
Alteromonas
antibiosis
marine bacteria
inhibitory substances
extracellular substance
Alteromonas
antibiosis
title_short Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
title_full Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
title_fullStr Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
title_full_unstemmed Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
title_sort Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
dc.creator.none.fl_str_mv León, Jorge
Tapia, Guillermina
Avalos, Rita
author León, Jorge
author_facet León, Jorge
Tapia, Guillermina
Avalos, Rita
author_role author
author2 Tapia, Guillermina
Avalos, Rita
author2_role author
author
dc.subject.none.fl_str_mv bacterias marinas
actividad inhibitoria
sustancia extracelular
Alteromonas
antibiosis
marine bacteria
inhibitory substances
extracellular substance
Alteromonas
antibiosis
topic bacterias marinas
actividad inhibitoria
sustancia extracelular
Alteromonas
antibiosis
marine bacteria
inhibitory substances
extracellular substance
Alteromonas
antibiosis
description A member of the genus Alteromonas sp. (strain N22.C) isolated from marine neuston and substance-producer which inhibit growth of ichthyopathogenics was characterized phenotipically by means of morphological, physiological and biochemical conventional tests. To determine the chemical nature of the inhibitory substance, raw extracts of supernatans from cultured strains were precipitated with increasing concentrations of Ammonium Sulphate up to 70% and filtered through columns of Sephadex G-25. Assays by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS–PAGE) revealed that the antimicrobial substance is a proteinaceous compound with a molecular mass of approximately 34000 Da, which lacks sugar associated residues of it. Other assays made with raw semipurified fractions extracts of Alteromonas sp N22.C show the wide spectrum of antibiotic activity against pathogenic bacterial strains of fish, mollusks and crustaceans.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2411
10.15381/rpb.v12i3.2411
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2411
identifier_str_mv 10.15381/rpb.v12i3.2411
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2411/2110
dc.rights.none.fl_str_mv Derechos de autor 2005 Jorge León, Guillermina Tapia, Rita Avalos
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2005 Jorge León, Guillermina Tapia, Rita Avalos
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 12 Núm. 3 (2005); 359-368
Revista Peruana de Biología; Vol. 12 No. 3 (2005); 359-368
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238309071421440
score 13.95948
Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.
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