Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics
Descripción del Articulo
Antibiotics are used in cryopreservation of stallion semen in order to control the growth of microorganisms; however they can have negative effects on the spermatozoa. This study aimed to evaluate the effect of two antibiotic preparations on microbial growth and quality of cryopreserved stallion sem...
| Autores: | , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2016 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/11650 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11650 |
| Nivel de acceso: | acceso abierto |
| Materia: | bacteria semen quality freezing fungi bacterias calidad seminal congelación hongos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics Proliferación Microbiana y Calidad Posdescongelación de Semen Equino Criopreservado en Presencia de Antibióticos |
| title |
Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics |
| spellingShingle |
Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics Giovanni Restrepo Betancur, Giovanni bacteria semen quality freezing fungi bacterias calidad seminal congelación hongos |
| title_short |
Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics |
| title_full |
Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics |
| title_fullStr |
Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics |
| title_full_unstemmed |
Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics |
| title_sort |
Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibiotics |
| dc.creator.none.fl_str_mv |
Giovanni Restrepo Betancur, Giovanni Rodríguez Mazo, Leonardo Duque Cortés, Juan Esteban |
| author |
Giovanni Restrepo Betancur, Giovanni |
| author_facet |
Giovanni Restrepo Betancur, Giovanni Rodríguez Mazo, Leonardo Duque Cortés, Juan Esteban |
| author_role |
author |
| author2 |
Rodríguez Mazo, Leonardo Duque Cortés, Juan Esteban |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
bacteria semen quality freezing fungi bacterias calidad seminal congelación hongos |
| topic |
bacteria semen quality freezing fungi bacterias calidad seminal congelación hongos |
| description |
Antibiotics are used in cryopreservation of stallion semen in order to control the growth of microorganisms; however they can have negative effects on the spermatozoa. This study aimed to evaluate the effect of two antibiotic preparations on microbial growth and quality of cryopreserved stallion semen. Semen from 10 Colombian Creole horses was cryopreserved by conventional freezing with two antibiotic preparations: P1 (penicillin 10000 UI/ml and streptomycin 10 mg/ml) and P2 (penicillin 10000 UI/ml, streptomycin 10 mg/ml, and amphotericin B 25 μg/ml) and a control group without antibiotics. After thawing, computerized sperm motility evaluation, hypoosmotic test (HOS) and counting of bacteria and fungi in semen were performed. Statistical analysis was done using generalized linear models (GLM) and comparison of means by Tukey. Significant difference (p<0.05) for total motility (MT%) between P1 (32.2 ± 17.6) and P2 (34.4 ± 19.1) with the control group (39.7 ± 16.5) was found, as well as for progressive motility (MP%) between P1 (15.5 ± 14.8) with P2 (17.9 ± 16.5) and control group (19.6 ± 14.1). For microbial growth, significant difference (p<0.05) for bacteria (CFU/ml) between P1 (167.7 ± 237.1) and P2 (240.1 ± 321.0) with the control group (464.4 ± 448.5) was found. Antibiotic preparations with penicillin, streptomycin and amphotericin B on stallion semen cryopreservation reduce bacterial growth but affect post-thaw sperm motility. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-06-15 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11650 10.15381/rivep.v27i2.11650 |
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https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11650 |
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10.15381/rivep.v27i2.11650 |
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spa |
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spa |
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https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11650/10653 |
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https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/4.0 |
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openAccess |
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application/pdf |
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Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
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Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
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Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 316-325 Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 316-325 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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1795238226794905600 |
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Microbial growth and post-thaw quality of stallion semen cryopreserved in presence of antibioticsProliferación Microbiana y Calidad Posdescongelación de Semen Equino Criopreservado en Presencia de AntibióticosGiovanni Restrepo Betancur, GiovanniRodríguez Mazo, LeonardoDuque Cortés, Juan Estebanbacteriasemen qualityfreezingfungibacteriascalidad seminalcongelaciónhongosAntibiotics are used in cryopreservation of stallion semen in order to control the growth of microorganisms; however they can have negative effects on the spermatozoa. This study aimed to evaluate the effect of two antibiotic preparations on microbial growth and quality of cryopreserved stallion semen. Semen from 10 Colombian Creole horses was cryopreserved by conventional freezing with two antibiotic preparations: P1 (penicillin 10000 UI/ml and streptomycin 10 mg/ml) and P2 (penicillin 10000 UI/ml, streptomycin 10 mg/ml, and amphotericin B 25 μg/ml) and a control group without antibiotics. After thawing, computerized sperm motility evaluation, hypoosmotic test (HOS) and counting of bacteria and fungi in semen were performed. Statistical analysis was done using generalized linear models (GLM) and comparison of means by Tukey. Significant difference (p<0.05) for total motility (MT%) between P1 (32.2 ± 17.6) and P2 (34.4 ± 19.1) with the control group (39.7 ± 16.5) was found, as well as for progressive motility (MP%) between P1 (15.5 ± 14.8) with P2 (17.9 ± 16.5) and control group (19.6 ± 14.1). For microbial growth, significant difference (p<0.05) for bacteria (CFU/ml) between P1 (167.7 ± 237.1) and P2 (240.1 ± 321.0) with the control group (464.4 ± 448.5) was found. Antibiotic preparations with penicillin, streptomycin and amphotericin B on stallion semen cryopreservation reduce bacterial growth but affect post-thaw sperm motility.Los antibióticos son utilizados en la criopreservación de semen equino con el fin de controlar la proliferación de microorganismos; sin embargo, estos pueden tener efectos negativos sobre los espermatozoides. La presente investigación tuvo como objetivo evaluar el efecto de dos preparaciones antibióticas sobre el desarrollo microbiano y la calidad de semen equino criopreservado. El semen de 10 caballos criollos colombianos fue criopreservado por congelación convencional, con dos preparaciones antibióticas: P1 (penicilina 10000 UI/ml y estreptomicina 10 mg/ml) y P2 (penicilina 10000 UI/ml, estreptomicina 10 mg/ml y anfotericina B 25 µg/ml) y un grupo control sin antibióticos. Posterior a la descongelación, se realizó la evaluación computarizada de la movilidad espermática, la prueba hipoosmótica (HOS) y el conteo de bacterias y hongos en el semen. El análisis estadístico se realizó mediante modelos lineales generalizados (GLM) y la comparación de medias por Tukey. Se encontró diferencia significativa (p<0.05) para movilidad total (MT%) entre P1 (32.2 ± 17.6) y P2 (34.4 ± 19.1) con el grupo control (39.7 ± 16.5) y para movilidad progresiva (MP%) entre P1 (15.5 ± 14.8) con P2 (17.9 ± 16.5) y el control (19.6 ± 14.1). Para la proliferación microbiana se encontró diferencia significativa (p<0.05) para bacterias (UFC/ml) entre P1 (167.7 ± 237.1) y P2 (240.1 ± 321.0) con el grupo control (464.4 ± 448.5). El uso de preparaciones antibióticas con penicilina, estreptomicina y anfotericina B en la criopreservación de semen equino reduce la proliferación de bacterias, pero produce una disminución en la movilidad espermática posdescongelación.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2016-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1165010.15381/rivep.v27i2.11650Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 316-325Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 316-3251682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11650/10653Derechos de autor 2016 Giovanni Giovanni Restrepo Betancur, Leonardo Rodríguez Mazo, Juan Esteban Duque Cortéshttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/116502017-02-20T16:35:40Z |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
